The arctic fox (Vulpes lagopus), an apex predator with an omnipresent distribution in the Arctic, is a potential source of intestinal parasites that may endanger people and pet animals such as dogs, thus posing a health risk. Non-invasive methods, such as coprology, are often the only option when studying wildlife parasitic fauna. However, the detection and identification of parasites are significantly enhanced when used in combination with methods of molecular biology. Using both approaches, we identified unicellular and multicellular parasites in faeces of arctic foxes and carcasses of sibling voles (Microtus levis) in Svalbard, where molecular methods are used for the first time. Six new species were detected in the arctic fox in Svalbard, Eucoleus aerophilus, Uncinaria stenocephala, Toxocara canis, Trichuris vulpis, Eimeria spp., and Enterocytozoon bieneusi, the latter never found in the arctic fox species before. In addition, only one parasite was found in the sibling vole in Svalbard, the Cryptosporidium alticolis, which has never been detected in Svalbard before.

• Klíčová slova
• Arctic fox, Coprology, Parasites, Sibling vole, Svalbard,
• MeSH
• Ancylostomatoidea izolace a purifikace   MeSH
• Arvicolinae parazitologie   MeSH
• cizopasní červi izolace a purifikace   MeSH
• Cryptosporidium izolace a purifikace   MeSH
• divoká zvířata parazitologie   MeSH
• Eimeria izolace a purifikace   MeSH
• feces parazitologie   MeSH
• hlístice izolace a purifikace   MeSH
• lišky parazitologie   MeSH
• parazitární nemoci střev veterinární   MeSH
• psi MeSH
• Toxocara canis izolace a purifikace   MeSH
• Trichuris izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Arktida MeSH
• Svalbard MeSH

Mice are used most often as a model for human toxocariasis caused by Toxocara canis larvae. Variety of symptoms developing during the infection reflects behaviour of the larvae, which are able to escape from the intestine and further invade and damage various host organs. In order to find an approach enabling observation on parasite behaviour in mouse in vivo, we used an epifluorescence method and a small animal imaging system (SAIS). Larvae of T. canis were labelled by carboxyfluorescein succinimidyl ester (CFSE) which incorporated on the parasite gastrointestinal tract. Following infection of BALB/c mice by CFSE-labelled larvae it has been observed that staining had no influence on viability and further migratory activity of the parasites through the host organs (the intestine, liver, lungs and brain) where they were detected by SAIS until day 17 p.i. In addition, the dye did not affect larval antigenic activity as well as the development of related immune response. Imaging of parasites labelled by CFSE, therefore, may represent a promising way to study behaviour of T. canis larvae in a paratenic host.

• MeSH
• anatomické struktury zvířat parazitologie   MeSH
• barvení a značení MeSH
• časové faktory MeSH
• fluoresceiny metabolismus   MeSH
• fluorescenční barviva metabolismus   MeSH
• modely nemocí na zvířatech MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• nemoci hlodavců parazitologie   MeSH
• sukcinimidy metabolismus   MeSH
• Toxocara canis růst a vývoj   izolace a purifikace   patogenita   MeSH
• toxokaróza parazitologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 5-(6)-carboxyfluorescein diacetate succinimidyl ester MeSH Prohlížeč
• fluoresceiny MeSH
• fluorescenční barviva MeSH
• sukcinimidy MeSH