Goeckerman therapy (GT) for psoriasis combines the therapeutic effect of crude coal tar (CCT) and ultraviolet radiation (UVR). CCT contains polycyclic aromatic hydrocarbons, some of which can form DNA adducts that may induce mutations and contribute to carcinogenesis. The aim of our work was to evaluate the relationship between concentrations of benzo[a]pyrene-7,8-diol-9,10-epoxide-DNA adducts (BPDE-DNA adducts) and rs4646903 (CYP1A1 gene), rs1048943 (CYP1A1), rs1056836 (CYP1B1), rs1051740 (EPHX1), rs2234922 (EPHX1) and rs8175347 (UGT1A1) polymorphic sites, and GSTM1 null polymorphism in 46 patients with chronic stable plaque psoriasis who underwent GT. The level of BPDE-DNA adducts was determined using the OxiSelect BPDE-DNA Adduct ELISA Kit. Polymerase chain reaction (PCR) and restriction fragment length polymorphism analysis (rs4646903, rs1048943, rs1051740, and rs2234922), fragment analysis (rs8175347), real-time PCR (rs1056836), and digital droplet PCR polymorphism (GSTM1) were used. CYP1B1*1/*1 wild-type subjects and CYP1B1*3/*1 heterozygotes for rs1056836 formed significantly higher amounts of BPDE-DNA adducts than CYP1B1*3/*3 homozygotes (p=0.031 and p=0.005, respectively). Regarding rs1051740, individuals with EPHX1*3/*1 heterozygosity revealed fewer adducts than EPHX1*1/*1 wild-type subjects (p=0.026). Our data suggest that CYP1B1/EPHX1 genotyping could help to predict the risk of DNA damage and to optimize doses of coal tar and UVR exposure in psoriatic patients in whom GT was applied.
- Klíčová slova
- BPDE-DNA adducts, Benzo[a]pyrene, Genetic polymorphisms, Psoriasis, Tar,
- MeSH
- 7,8-dihydro-7,8-dihydroxybenzo(a)pyren 9,10-oxid metabolismus MeSH
- adukty DNA metabolismus MeSH
- aplikace kožní MeSH
- benzopyren aplikace a dávkování škodlivé účinky metabolismus MeSH
- biotransformace MeSH
- cytochrom P450 CYP1B1 genetika metabolismus MeSH
- dehet uhelný aplikace a dávkování škodlivé účinky metabolismus MeSH
- dospělí MeSH
- epoxid hydrolasy genetika metabolismus MeSH
- farmakogenetika MeSH
- fenotyp MeSH
- frekvence genu MeSH
- glukuronosyltransferasa genetika metabolismus MeSH
- glutathiontransferasa genetika metabolismus MeSH
- heterozygot MeSH
- hodnocení rizik MeSH
- homozygot MeSH
- keratolytika aplikace a dávkování škodlivé účinky metabolismus MeSH
- kvantitativní polymerázová řetězová reakce MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- polymorfismus genetický * MeSH
- poškození DNA MeSH
- psoriáza enzymologie genetika terapie MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- terapie ultrafialovými paprsky * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 7,8-dihydro-7,8-dihydroxybenzo(a)pyren 9,10-oxid MeSH
- adukty DNA MeSH
- benzo(a)pyrene-7,8-dihydrodiol-9,10-epoxide-DNA MeSH Prohlížeč
- benzopyren MeSH
- CYP1B1 protein, human MeSH Prohlížeč
- cytochrom P450 CYP1B1 MeSH
- dehet uhelný MeSH
- EPHX1 protein, human MeSH Prohlížeč
- epoxid hydrolasy MeSH
- glukuronosyltransferasa MeSH
- glutathione S-transferase M1 MeSH Prohlížeč
- glutathiontransferasa MeSH
- keratolytika MeSH
- UGT1A1 enzyme MeSH Prohlížeč
