Although hypothermic treatment has been reported to have some beneficial effects on ischaemia at the clinical level, the mechanism of ischaemia suppression by hypothermia remains unclear due to a lack of mechanism understanding and insufficient data. The aim of this study was to isolate and characterize microRNAs specifically expressed in ischaemia-hypothermia for the dihydropyrimidinase-like 3 (Dpysl3) gene. PC12 cells were induced with CoCl2 for chemical ischaemia and incubated at 32 ℃ for hypothermia. In ischaemia-hypothermia, four types of microRNAs (miR-106b-5p, miR-194-5p, miR-326-5p, and miR-497-5p) were highly related to the Dpysl3 gene based on exosomal microRNA analysis. Dpysl3 gene expression was up-regulated by miR-497-5p but down-regulated by miR-106b-5p, miR-194-5p and miR-326-5p. Our results suggest that these four microRNAs are involved in the regulation of Dpysl3 gene expression. These findings provide valuable clues that exosomal microRNAs could be used as therapeutic targets for effective treatment of ischaemia.
- Klíčová slova
- PC12 cells, dihydropyrimidinase like 3 (Dpysl3), hypothermia, ischaemia, microRNAs,
- MeSH
- buňky PC12 MeSH
- exprese genu MeSH
- hypotermie * genetika MeSH
- ischemie chemicky indukované genetika MeSH
- krysa rodu Rattus MeSH
- lidé MeSH
- mikro RNA * genetika metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dihydropyrimidinase MeSH Prohlížeč
- Dpysl3 protein, rat MeSH Prohlížeč
- mikro RNA * MeSH
- MIRN326 microRNA, human MeSH Prohlížeč
- MIRN326 microRNA, rat MeSH Prohlížeč
- MIRN497 microRNA, human MeSH Prohlížeč
- MIRN497 microRNA, rat MeSH Prohlížeč
With high survival rates for chronic myeloid leukemia (CML) patients treated with BCR-ABL1 tyrosine kinase inhibitors (TKIs), emerging consequences, such as arterial ischemic events, require consideration when evaluating treatment options. Cardiovascular ischemic event incidence in clinical trials was evaluated in 2712 dasatinib-treated patients with Philadelphia chromosome-positive (Ph+) leukemias from 11 first- and second-line trials (pooled), newly diagnosed CML patients treated with dasatinib or imatinib (DASISION), and prostate cancer patients treated with dasatinib or placebo plus docetaxel/prednisone (READY). Overall, 2-4% of dasatinib-treated patients had cardiovascular ischemic events. Most dasatinib-treated patients with an event had a history of and/or risk factor for atherosclerosis (pooled 77 with history/risk and event/96 with events; DASISION 8/10; READY 15/18). Most cardiovascular ischemic events occurred within 1 year of initiating dasatinib (pooled 69/96; DASISION 7/10; READY 16/18). Comparison of observed and expected event rates through standardized incidence ratios indicates that dasatinib does not increase risk for cardiovascular ischemic events compared with external reference populations.
- Klíčová slova
- Cardiovascular, Chronic myeloid leukemia, Dasatinib, Ischemic, Tyrosine kinase inhibitors,
- MeSH
- chronická myeloidní leukemie farmakoterapie MeSH
- dasatinib škodlivé účinky MeSH
- dospělí MeSH
- hodnocení výsledků zdravotní péče metody statistika a číselné údaje MeSH
- incidence MeSH
- inhibitory proteinkinas škodlivé účinky MeSH
- ischemie chemicky indukované epidemiologie MeSH
- kardiovaskulární nemoci chemicky indukované epidemiologie MeSH
- klinické zkoušky jako téma metody statistika a číselné údaje MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádory prostaty farmakoterapie MeSH
- protokoly antitumorózní kombinované chemoterapie škodlivé účinky terapeutické užití MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- dasatinib MeSH
- inhibitory proteinkinas MeSH
Cyclosporin A is a basic immunosuppresive drug after organ transplantation. Morphological and functional features of Cyclosporin A nephrotoxicity caused by Sandimmune (Sandoz) and Consupren (of the Czech origin) were investigated in male Wistar rats. Rats were subjected to a right side nephrectomy followed by 45-minute- ischemia of remaining left kidney. Sandimmune was administered to one group of animals, Consupren to another group, both in the amount of 10 mg/kg/day. The second part of the experiment was performed in animals with right side nephrectomy only (without ischemia of the left side kidney) followed by the same administration of drugs. Changes were checked the 3rd and 21st day after nephrectomy. Ischemic arterial insudation lasted in the 3rd day set of animals with nephrectomy and left kidney ischemia treated by Consupren and was lacking after Sandimmune. Microvascularization of tubular epithelial cells was observed in significant frequency in the 21st day set of animals with unilateral nephrectomy without ischemia after Consupren and not after Sandimmune. The finding correlated with significantly higher blood level of Consupren and higher creatinine concentrations in serum than those of Sandimmune in rats with unilateral nephrectomy only.
- MeSH
- cyklosporin toxicita MeSH
- imunosupresiva toxicita MeSH
- ischemie chemicky indukované patologie MeSH
- krysa rodu Rattus MeSH
- ledviny krevní zásobení účinky léků ultrastruktura MeSH
- potkani Wistar MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- cyklosporin MeSH
- imunosupresiva MeSH
The authors discuss a case of the development of ischaemic colitis in a 69-year-old female patient with superficial thrombophlebitis of the lower extremity treated by a combination of dehydrated ergot alkaloid Secatoxin forte drops with smaller doses of Heparin inj. After ruling out other causes of the development of ischaemia, the authors consider ischaemic colitis a local manifestation of ergotism.
- MeSH
- ergotismus komplikace MeSH
- ischemie chemicky indukované MeSH
- kolitida chemicky indukované MeSH
- kolon krevní zásobení MeSH
- lidé MeSH
- senioři MeSH
- Check Tag
- lidé MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- kazuistiky MeSH
The development of pharmacotherapy implies great advantages for patients, at the same time it is, however, also the source of more frequent side-effects of drugs, which may result also from mutual interactions of commonly used drugs. The authors describe a case of ergotism which developed as a result of drug interaction between the ergot uterotonic Cornutamine and the marcolid antibiotic Erythromycin. This interaction could have seriously damaged the health of the young woman. At the same time the authors remind of the clinical picture of ergotism and the possible way of its treatment. In the Czechoslovak literature no report on this interaction was published during the past three years.
- MeSH
- akutní nemoc MeSH
- dospělí MeSH
- ergotamin škodlivé účinky MeSH
- ergotismus diagnóza etiologie MeSH
- erythromycin škodlivé účinky MeSH
- ischemie chemicky indukované MeSH
- končetiny krevní zásobení MeSH
- lékové interakce MeSH
- lidé MeSH
- poruchy v puerperiu chemicky indukované diagnóza MeSH
- těhotenství MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- těhotenství MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- kazuistiky MeSH
- Názvy látek
- ergotamin MeSH
- erythromycin MeSH
- MeSH
- dospělí MeSH
- ezofageální a žaludeční varixy komplikace MeSH
- gastrointestinální krvácení farmakoterapie etiologie MeSH
- ischemie chemicky indukované MeSH
- játra krevní zásobení MeSH
- lidé středního věku MeSH
- lidé MeSH
- lypresin škodlivé účinky terapeutické užití MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- lypresin MeSH
- MeSH
- amputace MeSH
- benzathin-penicilin G škodlivé účinky MeSH
- dítě MeSH
- injekce intramuskulární MeSH
- ischemie chemicky indukované MeSH
- lidé MeSH
- nekróza chemicky indukované MeSH
- prsty nohy MeSH
- revmatická horečka prevence a kontrola MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- Názvy látek
- benzathin-penicilin G MeSH
