The Candida albicans heat shock protein 90 kDa (hsp90-CA) is an important target for protective antibodies in disseminated candidiasis of experimental mice and humans. Hsp90-CA is present in the cell wall of Candida pseudohyphae or hyphae--typical pathogenic morphotypes in both mucosal and systemic Candida infections. However, the potential protective effects of hsp90-CA-specific antibodies in vaginal candidiasis has not yet been reported. In the present study we used various vaccine formulations (recombinant hsp90-CA protein and hsp90-CA-encoding DNA vaccine) and routes of administration (intradermal, intranasal, and intravenous) to induce both hsp90-CA-specific systemic and vaginal mucosa immune responses in experimental BALB/c mice. The results showed that intradermal recombinant hsp90-CA protein priming, followed by intranasal or intradermal recombinant hsp90-CA protein boosting induced significant increases in both serum and vaginal hsp90-CA-specific IgG and IgA antibodies compared to the control group, as well as enhanced hsp90-CA-specific splenocyte responses in vitro. In the intradermally boosted group, subsequent experimental vaginal Candida infection induced additional increases in the hsp90-CA specific IgG isotype, suggesting that Candida has the ability to induce a local hsp90-specific antibody (IgG) response during vulvovaginal candidiasis. Further work is required to elucidate the importance of immunity to highly conserved antigens during infection of the human female reproductive tract where a balance between immunity to and tolerance for commonly antigens such as hsp90 is necessary for the maintenance of fertility.
- MeSH
- aplikace intranazální MeSH
- Candida albicans imunologie MeSH
- DNA vakcíny aplikace a dávkování imunologie MeSH
- fungální proteiny imunologie MeSH
- fungální vakcíny aplikace a dávkování imunologie MeSH
- injekce intradermální MeSH
- injekce intravenózní MeSH
- kandidóza vulvovaginální imunologie MeSH
- lymfocyty imunologie MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- proliferace buněk MeSH
- proteiny tepelného šoku HSP90 imunologie MeSH
- protilátky fungální analýza krev MeSH
- sekundární imunizace MeSH
- slezina imunologie MeSH
- syntetické vakcíny aplikace a dávkování imunologie MeSH
- tvorba protilátek MeSH
- vagina imunologie mikrobiologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA vakcíny MeSH
- fungální proteiny MeSH
- fungální vakcíny MeSH
- proteiny tepelného šoku HSP90 MeSH
- protilátky fungální MeSH
- syntetické vakcíny MeSH
OBJECTIVE: This study was undertaken to characterize systemic immunity in the patients with recurrent vulvovaginal candidiasis. SUBJECT: Original prospective study. SETTING: 1. Department of Biological and Medical sciences, Charles University in Prague, Faculty of Pharmacy, Hradec Kralove, 2. Department of Obstetrics and Gynaecology, Medical Faculty, Hradec Kralove, Charles University in Prague, 3. Department of Clinical Immunology and Allergology, University Hospital, Hradec Kralove, 4. Department of Clinical Microbiology, University Hospital, Hradec Kralove, Czech republic. SUBJECT AND METHOD: Basic immunological parameters in 44 patients with recurrent vulvovaginal candidiasis were investigated and analyzed in this longitudinal follow-up study. CONCLUSION: Although there is no fundamental disturbance in systemic immunity in patients with recurrent vulvovaginal candidiasis and the majority of systemic immunological parameters in the patients were within normal range, some subtle changes of a few parameters of humoral and cellular immunity in contrast to the controls were identified.
- MeSH
- dospělí MeSH
- kandidóza vulvovaginální imunologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- recidiva MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
OBJECTIVE: A review of immunological aspects of fungal infections with focus on vulvovaginal candidiasis. DESIGN: Review. SETTING: Department of Obstetrics and Gynaecology, Medical Faculty, Hradec Kralove, Charles University in Prague, Department of Biological and Medical sciences, Faculty of Pharmacy, Hradec Kralove, Charles University in Prague, Department of Clinical Immunology and Allergology, University Hospital, Hradec Kralove, Department of Clinical Microbiology, University Hospital, Hradec Kralove. SUBJECT AND METHOD: Survey and discussion of current immunological aspects of vulvovaginal candidias based on literature review and personal experience. CONCLUSION: The current knowledge of immunity to Candida vulvovaginitis, particularly from experimental studies on animal models, support a more relevant role of local protection mechanisms than systemic immunity. As a consequence of this compartmentalization of the immune response, the role of innate and adaptive immunity remains unclear. The development of vulvovaginal candidiasis depends on a number of factors, including both the individual predisposition of a macroorganism and the properties of a fungal strain, and the environmental conditions influencing a delicate balance between the host and the yeast.
- MeSH
- chronická nemoc MeSH
- kandidóza vulvovaginální diagnóza imunologie terapie MeSH
- lidé MeSH
- recidiva MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
