Nejvíce citovaný článek - PubMed ID 10204613
Fusarium-derived mycotoxin deoxynivalenol (DON) usually induces diarrhea, vomiting and gastrointestinal inflammation. We studied the cytotoxic effect of DON on porcine small intestinal epithelium using the intestinal porcine epithelial cell line IPEC-J2. We screened out differentially expressed genes (DEGs) using RNA-seq and identified 320 upregulated genes and 160 downregulated genes. The enrichment pathways of these DEGs focused on immune-related pathways. DON induced proinflammatory gene expression, including cytokines, chemokines and other inflammation-related genes. DON increased IL1A, IL6 and TNF-α release and DON activated the phosphorylation of extracellular signal-regulated kinase-1 and-2 (ERK1/2), JUN N-terminal kinase (JNK) and p38 MAPK. A p38 inhibitor attenuated DON-induced IL6, TNF-α, CXCL2, CXCL8, IL12A, IL1A, CCL20, CCL4 and IL15 production, while an ERK1/2 inhibitor had only a small inhibitory effect on IL15 and IL6. An inhibitor of p38 MAPK decreased the release of IL1A, IL6 and TNF-α and an inhibitor of ERK1/2 partly attenuated protein levels of IL6. These data demonstrate that DON induces proinflammatory factor production in IPEC-J2 cells by activating p38 and ERK1/2.
- Klíčová slova
- IPEC-J2 cells, MAPKs, RNA-seq, deoxynivalenol, inflammation,
- MeSH
- buněčné linie MeSH
- epitelové buňky účinky léků imunologie metabolismus MeSH
- interleukin-1 genetika MeSH
- interleukin-6 genetika MeSH
- MAP kinasový signální systém účinky léků genetika imunologie MeSH
- mitogenem aktivované proteinkinasy p38 metabolismus MeSH
- prasata MeSH
- střevní sliznice účinky léků imunologie metabolismus MeSH
- TNF-alfa genetika MeSH
- transkriptom účinky léků MeSH
- trichotheceny toxicita MeSH
- viabilita buněk účinky léků MeSH
- zánět MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- deoxynivalenol MeSH Prohlížeč
- interleukin-1 MeSH
- interleukin-6 MeSH
- mitogenem aktivované proteinkinasy p38 MeSH
- TNF-alfa MeSH
- trichotheceny MeSH
