Cellular activities in the regulation of growth or adhesion/migration involve protein (lectin)-carbohydrate recognition at the cell surface. Members of the galectin family of endogenous lectins additionally bind distinct intracellular ligands. These interactions with protein targets explain the relevance of their nuclear and cytoplasmic presence. Expression profiling for galectins and accessible binding sites is a histochemical approach to link localization with cellular growth properties. Non-cross-reactive antibodies for the homodimeric (proto-type) galectins-1, -2 and -7 and the chimera-type galectin-3 (Gal-3) as well as the biotinylated lectins were tested. This analysis was performed with the FaDu squamous carcinoma cell line and long-term cultured human and porcine epidermal cells as models for malignant and normal cells of squamous cell epithelial origin. A set of antibodies was added for phenotypic cell characterization. Strong nuclear and cytoplasmic signals of galectins and the differential reactivity of labeled galectins support the notion of their individual properties. The length of the period of culture was effective in modulating marker expression. Cytochemical expression profiling is a prerequisite for the selection of distinct proteins for targeted modulation of gene expression as a step toward functional analysis.

• MeSH
• buněčná diferenciace fyziologie   MeSH
• buněčné jádro metabolismus   ultrastruktura   MeSH
• epitelové buňky metabolismus   MeSH
• fenotyp MeSH
• galektiny metabolismus   MeSH
• lidé MeSH
• molekuly buněčné adheze metabolismus   MeSH
• prasata MeSH
• růstové látky metabolismus   MeSH
• sacharidy fyziologie   MeSH
• signální transdukce fyziologie   MeSH
• spinocelulární karcinom metabolismus   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• galektiny MeSH
• molekuly buněčné adheze MeSH
• růstové látky MeSH
• sacharidy MeSH