Fifty Escherichia coli strains isolated from stool samples of 51 healthy children, 143 strains isolated from stool samples of 327 children with diarrhea and 24 strains isolated from stool samples of 21 children with suspected hemolytic uremic syndrome were examined for the presence of Shiga toxin-producing E. coli virulence factors (shiga toxin 1 and 2, intimin and enterohemolysin) and their genes. Vero-cell assay and latex agglutination were used for detection of Shiga toxin 1 and 2, TSB agar with washed erythrocytes was used for detection of enterohemolysin; genes encoding shiga toxin 1 and 2, intimin and enterohemolysin were detected using multiplex PCR. The presence of E. coli strains harboring genes encoding shiga toxin 1 and 2 (12 strains), intimin (34 strains) and enterohemolysin (12 strains) was demonstrated.

• MeSH
• Adhesins, Bacterial analysis   MeSH
• Child MeSH
• Escherichia coli chemistry   isolation & purification   MeSH
• Feces microbiology   MeSH
• Hemolysin Proteins analysis   MeSH
• Escherichia coli Infections microbiology   MeSH
• Humans MeSH
• Polymerase Chain Reaction MeSH
• Child, Preschool MeSH
• Escherichia coli Proteins * MeSH
• Shiga Toxins analysis   MeSH
• Carrier Proteins analysis   MeSH
• Check Tag
• Child MeSH
• Humans MeSH
• Child, Preschool MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Slovakia MeSH
• Names of Substances
• Adhesins, Bacterial MeSH
• eaeA protein, E coli MeSH Browser
• enterohemolysin MeSH Browser
• Hemolysin Proteins MeSH
• Escherichia coli Proteins * MeSH
• Shiga Toxins MeSH
• Carrier Proteins MeSH