Interactions of macrophages with epithelium represent one of the pathways involved in regulating local immune mechanisms. We studied the effect of cell-cell contact with an epithelial monolayer on the phenotype of macrophages. Human monocytes and THP-1 macrophages were co-cultured with monolayers of human bronchial epithelial cells (HBECs), the alveolar type II-like cell line A549, renal adenocarcinoma epithelial cells (RA), and the lung fibroblast strain HFL-1. The expression of CD11b, CD14, CD54, and HLA-DR was measured by immunocytochemistry and flow cytometry and showed epithelial cell induction of CD54 and HLA-DR in monocytes and of all antigens in THP-1 cells. Co-culture with fibroblasts did not change the phenotype of macrophages. Separation by a filter insert inhibited most of the effects. Culture supernatants did not induce prominent phenotypic changes. Cell-cell contacts with epithelium appear to be of importance in regulating the phenotype of macrophages.

• MeSH
• antigeny CD14 analýza   MeSH
• epitelové buňky imunologie   MeSH
• HLA-DR antigeny analýza   MeSH
• imunofenotypizace MeSH
• kokultivační techniky MeSH
• ledviny cytologie   MeSH
• lidé MeSH
• makrofágový antigen 1 analýza   MeSH
• makrofágy imunologie   MeSH
• mezibuněčná adhezivní molekula-1 analýza   MeSH
• mezibuněčná komunikace imunologie   MeSH
• monocyty imunologie   MeSH
• nádorové buňky kultivované MeSH
• plíce cytologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, P.H.S. MeSH
• Názvy látek
• antigeny CD14 MeSH
• HLA-DR antigeny MeSH
• makrofágový antigen 1 MeSH
• mezibuněčná adhezivní molekula-1 MeSH