Specific primers for the detection of the FIV provirus in peripheral blood mononuclear cells (PBMC) by seminested PCR (snPCR) were developed. Forty cats (patients from veterinary hospitals) were investigated for the presence of FIV serum antibodies by immunoblot and for the presence of provirus in PBMC by snPCR. Seventeen of the 40 examined samples (42.5%) showed the presence of antibodies against FIV. The total number of animals that were found positive in snPCR was 19 (47.5%). Fourteen of the seropositive animals (35%) were positive by snPCR whereas three seropositive animals (7.5%) turned out to be snPCR negative. Of twenty-three animals that were negative by immunoblot, five (12.5%) were found to be positive by snPCR.

• MeSH
• DNA virů analýza   MeSH
• imunoblotting MeSH
• kočky mikrobiologie   MeSH
• lentivirové infekce veterinární   MeSH
• leukocyty mononukleární virologie   MeSH
• nemoci koček imunologie   mikrobiologie   MeSH
• polymerázová řetězová reakce veterinární   MeSH
• protilátky virové krev   MeSH
• proviry genetika   izolace a purifikace   MeSH
• senzitivita a specificita MeSH
• virus kočičí imunodeficience genetika   imunologie   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• kočky mikrobiologie   MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• DNA virů MeSH
• protilátky virové MeSH