Detection of feline immunodeficiency provirus by seminested polymerase chain reaction
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
11271826
DOI
10.1007/bf02817417
Knihovny.cz E-zdroje
- MeSH
- DNA virů analýza MeSH
- imunoblotting MeSH
- kočky mikrobiologie MeSH
- lentivirové infekce veterinární MeSH
- leukocyty mononukleární virologie MeSH
- nemoci koček imunologie mikrobiologie MeSH
- polymerázová řetězová reakce veterinární MeSH
- protilátky virové krev MeSH
- proviry genetika izolace a purifikace MeSH
- senzitivita a specificita MeSH
- virus kočičí imunodeficience genetika imunologie izolace a purifikace MeSH
- zvířata MeSH
- Check Tag
- kočky mikrobiologie MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- DNA virů MeSH
- protilátky virové MeSH
Specific primers for the detection of the FIV provirus in peripheral blood mononuclear cells (PBMC) by seminested PCR (snPCR) were developed. Forty cats (patients from veterinary hospitals) were investigated for the presence of FIV serum antibodies by immunoblot and for the presence of provirus in PBMC by snPCR. Seventeen of the 40 examined samples (42.5%) showed the presence of antibodies against FIV. The total number of animals that were found positive in snPCR was 19 (47.5%). Fourteen of the seropositive animals (35%) were positive by snPCR whereas three seropositive animals (7.5%) turned out to be snPCR negative. Of twenty-three animals that were negative by immunoblot, five (12.5%) were found to be positive by snPCR.
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