Detection of feline immunodeficiency provirus by seminested polymerase chain reaction
Language English Country United States Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
11271826
DOI
10.1007/bf02817417
Knihovny.cz E-resources
- MeSH
- DNA, Viral analysis MeSH
- Immunoblotting MeSH
- Cats microbiology MeSH
- Lentivirus Infections veterinary MeSH
- Leukocytes, Mononuclear virology MeSH
- Cat Diseases immunology microbiology MeSH
- Polymerase Chain Reaction veterinary MeSH
- Antibodies, Viral blood MeSH
- Proviruses genetics isolation & purification MeSH
- Sensitivity and Specificity MeSH
- Immunodeficiency Virus, Feline genetics immunology isolation & purification MeSH
- Animals MeSH
- Check Tag
- Cats microbiology MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- DNA, Viral MeSH
- Antibodies, Viral MeSH
Specific primers for the detection of the FIV provirus in peripheral blood mononuclear cells (PBMC) by seminested PCR (snPCR) were developed. Forty cats (patients from veterinary hospitals) were investigated for the presence of FIV serum antibodies by immunoblot and for the presence of provirus in PBMC by snPCR. Seventeen of the 40 examined samples (42.5%) showed the presence of antibodies against FIV. The total number of animals that were found positive in snPCR was 19 (47.5%). Fourteen of the seropositive animals (35%) were positive by snPCR whereas three seropositive animals (7.5%) turned out to be snPCR negative. Of twenty-three animals that were negative by immunoblot, five (12.5%) were found to be positive by snPCR.
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J Vet Med Sci. 1997 Sep;59(9):739-46 PubMed
Am J Vet Res. 1993 Nov;54(11):1858-62 PubMed
J Virol Methods. 1992 Mar;36(3):239-48 PubMed
Cornell Vet. 1992 Oct;82(4):359-69 PubMed
J Virol. 1992 Jul;66(7):4040-9 PubMed
Vet Microbiol. 1994 Mar;39(1-2):153-65 PubMed
C R Acad Sci III. 1994 Dec;317(12):1123-34 PubMed
J Infect Dis. 1992 Sep;166(3):611-6 PubMed
N Engl J Med. 1991 Oct 24;325(17):1250-1 PubMed
Proc Natl Acad Sci U S A. 1989 Aug;86(15):5743-7 PubMed
Folia Microbiol (Praha). 1999;44(5):467-86 PubMed
J Virol Methods. 1992 Jun;37(3):241-52 PubMed
Clin Microbiol Rev. 1995 Jan;8(1):87-112 PubMed
Science. 1987 Feb 13;235(4790):790-3 PubMed
Zentralbl Veterinarmed B. 1999 Dec;46(10):665-71 PubMed
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