This work describes the first confirmed cases of carp oedema virus disease (CEVD) in Slovakia and the Czech Republic and the phylogenetic analysis of Czech and Slovak carp oedema virus (CEV) isolates. Four cases of disease outbreak in the Czech Republic are described, the oldest dating from mid-May 2013 and one case from Slovakia dating from May 2019. In all cases, virus presence was confirmed using nested PCR. PCR products were sequenced and compared with 357-bp nucleotide sequences encoding the CEV P4a protein in GenBank. In four cases of disease outbreak (three common carp breeding facilities and one koi garden pond), CEV detected belonged to genogroup I. In one case (koi garden pond), fish were confirmed as infected with CEV from genogroup II. This work complements data on CEV occurrence in European countries and contributes to a better understanding of the pathways leading to transmission of the virus throughout Europe.

Department of Ecology and Diseases of Zoo Animals Game Fish and Bees Faculty of Veterinary Hygiene and Ecology University of Veterinary and Pharmaceutical Sciences Brno Brno Czech Republic

Department of Zoology Fisheries Hydrobiology and Apiculture Mendel University in Brno Brno Czech Republic

Faculty of Fisheries and Protection of Waters South Bohemian Research Centre of Aquaculture and Biodiversity of Hydrocenoses University of South Bohemia in České Budějovice Ceske Budejovice Czech Republic

Veterinary Research Institute Brno Brno Czech Republic

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Adamek, M., Baska, F., Vincze, B., & Steinhagen, D. (2018). Carp edema virus from three genogroups is present in common carp in Hungary. Journal of Fish Diseases, 41(3), 463-468. https://doi.org/10.1111/jfd.12744

Adamek, M., Jung-Schroers, V., Hellmann, J., Teitge, F., Bergmann, S. M., Runge, M., … Steinhagen, D. (2016). Concentration of carp edema virus (CEV) DNA in koi tissues affected by koi sleepy disease (KSD). Diseases of Aquatic Organisms, 119, 245-251. https://doi.org/10.3354/dao02994

Adamek, M., Oschilewski, A., Wohlsein, P., Jung-Schroers, V., Teitge, F., Dawson, A., … Steinhagen, D. (2017). Experimental infections of different carp strains with the carp edema virus (CEV) give insights into the infection biology of the virus and indicate possible solutions to problems caused by koi sleepy disease (KSD) in carp aquaculture. Veterinary Research, 48, 12-28. https://doi.org/10.1186/s13567-017-0416-4

Bercovier, H., Fishman, Y., Nahary, R., Sinai, S., Zlotkin, A., Eyngor, M., … Hedrick, R. P. (2005). Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiology, 5, 13. https://doi.org/10.1186/1471-2180-5-13

Haenen, O., Way, K., Gorgoglione, B., Ito, T., Paley, R., Bigarré, L., & Waltzech, T. (2016). Novel viral infection threatening Cyprinid fish. Bulletin- European Association of Fish Pathology, 36(1), 11-23.

Haenen, O., Way, K., Stone, D., & Engelsma, M. (2014). Koi sleepy disease found for the first time in koi carps in the Netherlands. [in Dutch]. Tijdschrift Voor Diergeneeskunde, 139, 26-29.

Hedrick, R. P., Antonio, D. B., & Munn, R. J. (1997). Poxvirus like agent associated with epizootic mortality in juvenile koi (Cyprinus carpio). Frankston High School Newsletter, 25, 1-2.

Jung-Schroers, V., Adamek, M., Teitge, F., Hellmann, J., Bergmann, S. M., Schutze, H., … Steinhagen, D. (2015). Another potential carp killer?: Carp Edema Virus disease in Germany. BMC Veterinary Research, 11, 114. https://doi.org/10.1186/s12917-015-0424-7

Jung-Schroers, V., Heling, M., Adamek, M., Teitge, F., Bauer, J., Kleingeld, D. W., … Steinhagen, D. (2019). Epidemiological Study on the Occurrence and the Pathogenicity of the Carp Edema Virus (CEV) in Fish in Germany. Aquaculture Europe 19, Berlin, 7-10th October 2019, poster No 405, p. 648, abstract only.

Kim, S. W., Jun, J. W., Giri, S. S., Chi, C., Yun, S., Kim, H. J., … Park, S. C. (2017). First report of carp oedema virus infection of koi (Cyprinus carpio haematoptherus) in the Republic of Korea. Transboundary and Emerging Diseases, 65, 315-320. https://doi.org/10.1111/tbed.12782

Lewisch, E., Gorgoglione, B., Way, K., & El-Matbouli, M. (2015). Carp edema virus/koi sleepy disease: An emerging disease in central-east Europe. Transboundary and Emerging Diseases, 62, 6-12. https://doi.org/10.1111/tbed.12293

Lovy, J., Firend, S. E., Al-Hussinee, L., & Waltzek, T. B. (2018). First report of carp edema virus in the mortality of wild common carp Cyprinus carpio in North America. Diseases of Aquatic Organisms, 131, 177-186. https://doi.org/10.3354/dao03296

Matras, M., Borzym, E., Stone, D., Way, K., Stachnik, M., Maj-Paluch, J., … Reichert, M. (2017). Carp edema virus in Polish aquaculture - evidence of significant sequence divergence and a new lineage in common carp Cyprinus carpio (L.). Journal of Fish Diseases, 40, 319-325. https://doi.org/10.1111/jfd.12518

Matras, M., Stachnik, M., Borzym, E., Maj-Paluch, J., & Reichert, M. (2019). Potential vector species of carp edema virus (CEV). Journal of Fish Diseases, 42(7), 959-964. https://doi.org/10.1111/jfd.13000

Murakami, Y., Shitanaka, M., Toshida, S., & Matsuzato, T. (1976). Studies on mass mortality of juvenile carp: About mass mortality showing edema [in. Japanese]. Bulletin Hiroshima Fresh Water Fisheries Experimental Station, 19-33.

Ouyang, P., Yang, R., Chen, J., Wang, K., Geng, Y., Lai, W., … Yin, L. (2018). First detection of carp edema virus in association with cyprinid herpesvirus 3 in cultured ornamental koi, Cyprinus carpio L., in China. Aquaculture, 490, 162-168. https://doi.org/10.1016/j.aquaculture.2018.02.037

Oyamatsu, T., Matoyama, H., Yamamoto, K., & Fukuda, H. (1997). A trial for detection of carp edema virus by using polymerase chain reaction. Suisan Zoshoku, 45, 247-251. https://doi.org/10.11233/aquaculturesci1953.45.247

Radosavljevic, V., Adamek, M., Milicevic, V., Maksimovic-Zoric, J., & Steinhagen, D. (2018). Occurrence of two novel viral pathogens (CEV and CyHV-2) affecting Serbian cyprinid aquaculture and ichthyofauna. Journal of Fish Diseases, 41, 851-854. https://doi.org/10.1111/jfd.12789

Stevens, B. N., Michel, A., Liepnieks, M. L., Kenelty, K., Gardhouse, S. M., Groff, J. M., … Soto, E. (2018). Outbreak and treatment of carp edema virus in koi (Cyprinus carpio) from Northern California. Journal of Zoo and Wildlife Medicine, 49(3), 755-764. https://doi.org/10.1638/2017-0189-1

Swaminathan, T. R., Kumar, R., Dharmaratnam, A., Basheer, V. S., Sood, N., Pradhan, P. K., … Jena, J. K. (2016). Emergence of carp edema virus in cultured ornamental koi carp, Cyprinus carpio koi, in India. Journal of General Virology, 97, 3392-3399. https://doi.org/10.1099/jgv.0.000649

Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular evolutionary genetics analysis version 6.0. Molecular Biology and Evolution, 30(12), 2725-2729. https://doi.org/10.1093/molbev/mst197

Vesely, T., Pokorova, D., Reschova, S., & Piačkova, V. (2015). Detection of carp edema virus in common carp (Cyprinus carpio) and koi carp in the Czech Republic. EAFP 17th International Conference on disease of fish and shellfish, Las Palmas de Gran Canaria 7-11 September 2015. Poster P-122, European Association of Fish Pathologists, p 346 (abstract only).

Way, K., Haenen, O., Stone, D., Adamek, M., Bergmann, S. M., Bigarré, L., … Waltzek, T. (2017). Emergence of carp edema virus (CEV) and its significance to European common carp and koi Cyprinus carpio. Diseases of Aquatic Organisms, 126, 155-166. https://doi.org/10.3354/dao03164

Way, K., Martin, P., Mölkänen, S., Wood, G., Reading, A., Williams, C., & Stone, D. (2014). Detection of Carp Edema-like Virus during Disease Outbreaks in Koi and Common Carp (Cyprinus carpio) in the UK and examination of archive samples. In: Abstract book of 9th. International Symposium on Viruses of Lower Vertebrates, Malaga, October 1-4, 2014.

Way, K., & Stone, D. (2013). Emergence of carp edema virus-like (CEV-like) disease in the UK. Finfish News, 15, 32-34.

Way, K., Stone, D., Stinton, N., Gardiner, R., Wood, G., & Feist, S. (2013). Detection of Carp Edema-like Virus during Disease Outbreaks in Koi and Common Carp (Cyprinus carpio) in the UK. In: Abstract Book of 16th International Conference on Diseases of Fish and Shellfish, Tampere, September 2 - 6, 2013. O-028, abstract p. 39.

Zhang, X., Ni, Y., Ye, J., Xu, H., Hou, Y., Luo, W., & Shen, W. (2017). Carp edema virus, an emerging threat to the carp (Cyprinus carpio) industry in China. Aquaculture, 474, 34-39. 10/1016/j.aquaculture.201703.033

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