The double-disk synergy test (DDST) using Mueller-Hinton agar and antibiotic disks with centrally positioned disks of amoxicillin-clavulanate, ampicillin-sulbactam, and piperacillin-tazobactam and, at a center-to-center distance of 25-30 mm, 2-4 disks with 10 various beta-lactam antibiotics per one plate was performed in 58 clinical isolates of Stenotrophomonas maltophilia to determine the effectivity of 3 beta-lactamase inhibitors. When tested with clavulanate as the central beta-lactamase inhibitor synergic action on tested strains was the most frequent with aztreonam (81.0% of strains), cefoperazone (63.8%), and cefepime (60.3%). With sulbactam the synergic action, i.e. DDST positivity, was high in the case of cefoperazone (15.5%), ampicillin, aztreonam and piperacillin (8.6% each); with tazobactam it was the most frequent with aztreonam (53.4%), cefoperazone (44.8%) and cefepime (37.9%). No synergy was demonstrated after application of meropenem regardless of the kind of beta-lactamase inhibitor used. In 58 strains of S. maltophilia, 55 different profiles of DDST positivity were found. The results confirm that clavulanate is the most effective inhibitor of S. maltophilia beta-lactamases. The utilization of DDST (performed in the recommended way) for the typization of strains Stenotrophomonas species and for the estimation of potential effectiveness combinations of beta-lactams with beta-lactamase inhibitors for the therapy of stenotrophomonade infections was suggested.

• MeSH
• ampicilin farmakologie   MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence MeSH
• beta-laktamy farmakologie   MeSH
• inhibitory beta-laktamasy * MeSH
• inhibitory enzymů farmakologie   MeSH
• kombinace amoxicilinu a kyseliny klavulanové farmakologie   MeSH
• kombinace léků piperacilin a tazobactam MeSH
• kyselina penicilanová analogy a deriváty   farmakologie   MeSH
• mikrobiální testy citlivosti metody   MeSH
• piperacilin farmakologie   MeSH
• Stenotrophomonas maltophilia růst a vývoj   izolace a purifikace   metabolismus   MeSH
• sulbaktam farmakologie   MeSH
• synergismus léků MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• ampicilin MeSH
• antibakteriální látky MeSH
• beta-laktamy MeSH
• inhibitory beta-laktamasy * MeSH
• inhibitory enzymů MeSH
• kombinace amoxicilinu a kyseliny klavulanové MeSH
• kombinace léků piperacilin a tazobactam MeSH
• kyselina penicilanová MeSH
• piperacilin MeSH
• sulbaktam MeSH
• sultamicillin MeSH Prohlížeč

Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MIC at 37 than at 30 degrees C) were described. Changes in the distribution of saturated and unsaturated fatty acids (FA) at 30 and 37 degrees C were considered as one of possible causes of the TDS phenomenon. Gas chromatography was used to determine the distribution of individual FA in five 37TDS strains of S. maltophilia (Group I); in five strains with MIC values unaffected by the cultivation temperature (Group II) and in six 30TDS (four strains) or 30/37TDS (two strains) isolates (Group III). At identical temperatures, no statistically significant differences in the distribution of major FA (iso-15:0, anteiso-15:0, 16:0 and 16:1) were registered between individual groups. Statistically significant (p < 0.05) differences between groups were found in minor FA only (iso-16:0, iso-17:0 and iso-17:1). Distribution changes of cellular FA at 30 and 37 degrees C can be considered to play only a minor role in the formation of the TDS phenomenon.

• MeSH
• antibakteriální látky farmakologie   MeSH
• kyseliny mastné neesterifikované metabolismus   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• Stenotrophomonas maltophilia účinky léků   metabolismus   MeSH
• teplota MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• kyseliny mastné neesterifikované MeSH