This is the first evidence that replicating vectors can be successfully used for transient protein expression in BY-2 plant cell packs. Transient recombinant protein expression in plants and recently also plant cell cultures are of increasing interest due to the speed, safety and scalability of the process. Currently, studies are focussing on the design of plant virus-derived vectors to achieve higher amounts of transiently expressed proteins in these systems. Here we designed and tested replicating single and multi-cassette vectors that combine elements for enhanced replication and hypertranslation, and assessed their ability to express and particularly co-express proteins by Agrobacterium-mediated transient expression in tobacco BY-2 plant cell packs. Substantial yields of green and red fluorescent proteins of up to ~ 700 ng/g fresh mass were detected in the plant cells along with position-dependent expression. This is the first evidence of the ability of replicating vectors to transiently express proteins in BY-2 plant cell packs.

• Klíčová slova
• DsRed, GFP, Plant cell pack, Plant expression vector, Tobacco BY-2 cells, Transient co-expression,
• MeSH
• Agrobacterium genetika   MeSH
• buněčné kultury MeSH
• červený fluorescenční protein MeSH
• genetické vektory * MeSH
• geneticky modifikované rostliny genetika   MeSH
• luminescentní proteiny genetika   MeSH
• proteinové inženýrství metody   MeSH
• rekombinantní proteiny genetika   metabolismus   MeSH
• replikon MeSH
• rostlinné buňky metabolismus   MeSH
• tabák cytologie   genetika   MeSH
• zelené fluorescenční proteiny MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• luminescentní proteiny MeSH
• rekombinantní proteiny MeSH
• zelené fluorescenční proteiny MeSH