BACKGROUND: Pentosidine, an advanced glycation end product, increasingly accumulates in articular cartilage with age, and contributes to the pathogenesis of osteoarthritis (OA). Increased pentosidine concentrations are associated with inflammatory disorders-for example, rheumatoid arthritis. OBJECTIVE: To compare pentosidine serum concentrations in patients with knee OA and in healthy volunteers and to determine a relationship between pentosidine and cartilage oligomeric matrix protein (COMP)-a marker of articular cartilage destruction. METHODS: Paired serum and synovial fluid samples were obtained by arthrocentesis from 38 patients with knee OA and from 38 healthy volunteers. Pentosidine concentration was measured by reverse phase high performance liquid chromatography with fluorescent detection and COMP was determined by sandwich ELISA. RESULTS: Significantly increased serum pentosidine (p<0.01) and COMP (p<0.05) levels were detected in the patients with OA compared with the control group. Serum pentosidine correlated significantly with synovial fluid pentosidine (p<0.001). Pentosidine in synovial fluid (p<0.05) and in serum (p<0.05) correlated significantly with synovial fluid COMP. Pentosidine and COMP concentrations did not correlate significantly with the radiological stage of the disease. CONCLUSION: Increased pentosidine serum concentration in patients with OA and its correlation with the cartilage destruction marker COMP in synovial fluid suggests that pentosidine may be important in OA pathology and is a new potential OA marker.

• MeSH
• arginin analogy a deriváty   analýza   krev   MeSH
• artróza kolenních kloubů krev   diagnostické zobrazování   metabolismus   MeSH
• biologické markery analýza   krev   MeSH
• chrupavkový oligomerní matrixový protein MeSH
• extracelulární matrix - proteiny analýza   MeSH
• glykoproteiny analýza   MeSH
• kloubní chrupavka chemie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• lysin analogy a deriváty   analýza   krev   MeSH
• matriliny MeSH
• průřezové studie MeSH
• radiografie MeSH
• senioři MeSH
• stupeň závažnosti nemoci MeSH
• synoviální tekutina chemie   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• arginin MeSH
• biologické markery MeSH
• chrupavkový oligomerní matrixový protein MeSH
• extracelulární matrix - proteiny MeSH
• glykoproteiny MeSH
• lysin MeSH
• matriliny MeSH
• pentosidine MeSH Prohlížeč
• TSP5 protein, human MeSH Prohlížeč