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Nejvíce citované: 10974122

4 citací v PubMed Filtry

Nejvíce citovaný článek - PubMed ID 10974122

Záznam nenalezen v Medvik. Navštivte PubMed 10974122

Článek

Crystallographic fragment screening-based study of a novel FAD-dependent oxidoreductase from Chaetomium thermophilum

Švecová, Leona
Autor Švecová, Leona ORCID Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Průmyslová 595, 252 50 Vestec, Czech Republic
Østergaard, Lars Henrik
Autor Østergaard, Lars Henrik ORCID Novozymes A/S, Biologiens Vej 2, 2800 Kgs. Lyngby, Denmark
Skálová, Tereza
Autor Autorita ORCID Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Průmyslová 595, 252 50 Vestec, Czech Republic
Schnorr, Kirk Matthew
Autor Schnorr, Kirk Matthew ORCID Novozymes A/S, Biologiens Vej 2, 2800 Kgs. Lyngby, Denmark
Koval', Tomáš
Autor Koval', Tomáš ORCID Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Průmyslová 595, 252 50 Vestec, Czech Republic
Kolenko, Petr
Autor Autorita ORCID Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Průmyslová 595, 252 50 Vestec, Czech Republic
Stránský, Jan
Autor Stránský, Jan ORCID Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Průmyslová 595, 252 50 Vestec, Czech Republic
Sedlák, David
Autor Autorita ORCID CZ-OPENSCREEN: National Infrastructure for Chemical Biology, Institute of Molecular Genetics of the Czech Academy of Sciences, v.v.i., Vídeňská 1083, 142 20 Prague, Czech Republic
Dušková, Jarmila
Autor Dušková, Jarmila ORCID Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Průmyslová 595, 252 50 Vestec, Czech Republic
Trundová, Mária
Autor Trundová, Mária ORCID Institute of Biotechnology of the Czech Academy of Sciences, v.v.i., Průmyslová 595, 252 50 Vestec, Czech Republic

Acta crystallographica. Section D, Structural biology. 2021 Jun 01 ; 77 (Pt 6) : 755-775. [epub] 20210514

Acta Crystallogr D Struct Biol
ISSN 2059-7983
Zdroj

The FAD-dependent oxidoreductase from Chaetomium thermophilum (CtFDO) is a novel thermostable glycoprotein from the glucose-methanol-choline (GMC) oxidoreductase superfamily. However, CtFDO shows no activity toward the typical substrates of the family and high-throughput screening with around 1000 compounds did not yield any strongly reacting substrate. Therefore, protein crystallography, including crystallographic fragment screening, with 42 fragments and 37 other compounds was used to describe the ligand-binding sites of CtFDO and to characterize the nature of its substrate. The structure of CtFDO reveals an unusually wide-open solvent-accessible active-site pocket with a unique His-Ser amino-acid pair putatively involved in enzyme catalysis. A series of six crystal structures of CtFDO complexes revealed five different subsites for the binding of aryl moieties inside the active-site pocket and conformational flexibility of the interacting amino acids when adapting to a particular ligand. The protein is capable of binding complex polyaromatic substrates of molecular weight greater than 500 Da.

Klíčová slova
Chaetomium thermophilum, FAD-dependent oxidoreductases, GMC oxidoreductases, crystallographic fragment screening,
MeSH
Chaetomium enzymologie MeSH
flavinadenindinukleotid chemie MeSH
fungální proteiny chemie MeSH
konformace proteinů MeSH
molekulární modely * MeSH
oxidoreduktasy chemie MeSH
vazebná místa MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
flavinadenindinukleotid MeSH
fungální proteiny MeSH
oxidoreduktasy MeSH

Článek

Diversity and composition of the North Sikkim hot spring mycobiome using a culture-independent method

Folia microbiologica. 2021 Jun ; 66 (3) : 457-468. [epub] 20210323

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Zdroj

Fungi are considered to be the most resilient and economically important microbial community that can easily survive and optimally grow under a wide range of growth conditions. Thermophilic fungi from the geothermal sources have been less pondered upon and lie unexplored. Here, a microbiome approach was conducted to understand the concealed world of the environmental mycobiota from the two hot springs of North Sikkim district located in North-east India. The solfataric muds from the hot springs were analyzed. In both the samples, on the basis of genus level classification, genus Fusarium had the highest abundance followed by Colletotrichum, Pochonia, Pyricularia, Neurospora, etc. Analyzing the predicted genes, the functional proteins of New Yume Samdung mycobiome were found to be dominated by the genera Fusarium (22%), Trichoderma (12%), and Aspergillus (11%), whereas in the case of Old Yume Samdung, it was dominated by the genera Aspergillus (11%), Saccharomyces (6%), and Fusarium (5%). Interestingly, in the studied mycobiome, environmental yeasts were also detected. From the functional metagenomics, sulfate adenylatetransferase (SAT) proteins for sulfur assimilation were found in some of the fungal reads. Toxin protein reads such as AM-toxin biosynthesis proteins, AF-toxin biosynthesis proteins, Gliotoxin biosynthesis proteins, and aflatoxin biosynthesis proteins were detected in the mycobiomes.

Článek

Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species

Studies in mycology. 2020 Mar ; 95 () : 5-169. [epub] 20200627

Stud Mycol
ISSN 0166-0616
Zdroj

The Eurotiales is a relatively large order of Ascomycetes with members frequently having positive and negative impact on human activities. Species within this order gain attention from various research fields such as food, indoor and medical mycology and biotechnology. In this article we give an overview of families and genera present in the Eurotiales and introduce an updated subgeneric, sectional and series classification for Aspergillus and Penicillium. Finally, a comprehensive list of accepted species in the Eurotiales is given. The classification of the Eurotiales at family and genus level is traditionally based on phenotypic characters, and this classification has since been challenged using sequence-based approaches. Here, we re-evaluated the relationships between families and genera of the Eurotiales using a nine-gene sequence dataset. Based on this analysis, the new family Penicillaginaceae is introduced and four known families are accepted: Aspergillaceae, Elaphomycetaceae, Thermoascaceae and Trichocomaceae. The Eurotiales includes 28 genera: 15 genera are accommodated in the Aspergillaceae (Aspergillago, Aspergillus, Evansstolkia, Hamigera, Leiothecium, Monascus, Penicilliopsis, Penicillium, Phialomyces, Pseudohamigera, Pseudopenicillium, Sclerocleista, Warcupiella, Xerochrysium and Xeromyces), eight in the Trichocomaceae (Acidotalaromyces, Ascospirella, Dendrosphaera, Rasamsonia, Sagenomella, Talaromyces, Thermomyces, Trichocoma), two in the Thermoascaceae (Paecilomyces, Thermoascus) and one in the Penicillaginaceae (Penicillago). The classification of the Elaphomycetaceae was not part of this study, but according to literature two genera are present in this family (Elaphomyces and Pseudotulostoma). The use of an infrageneric classification system has a long tradition in Aspergillus and Penicillium. Most recent taxonomic studies focused on the sectional level, resulting in a well-established sectional classification in these genera. In contrast, a series classification in Aspergillus and Penicillium is often outdated or lacking, but is still relevant, e.g., the allocation of a species to a series can be highly predictive in what functional characters the species might have and might be useful when using a phenotype-based identification. The majority of the series in Aspergillus and Penicillium are invalidly described and here we introduce a new series classification. Using a phylogenetic approach, often supported by phenotypic, physiologic and/or extrolite data, Aspergillus is subdivided in six subgenera, 27 sections (five new) and 75 series (73 new, one new combination), and Penicillium in two subgenera, 32 sections (seven new) and 89 series (57 new, six new combinations). Correct identification of species belonging to the Eurotiales is difficult, but crucial, as the species name is the linking pin to information. Lists of accepted species are a helpful aid for researchers to obtain a correct identification using the current taxonomic schemes. In the most recent list from 2014, 339 Aspergillus, 354 Penicillium and 88 Talaromyces species were accepted. These numbers increased significantly, and the current list includes 446 Aspergillus (32 % increase), 483 Penicillium (36 % increase) and 171 Talaromyces (94 % increase) species, showing the large diversity and high interest in these genera. We expanded this list with all genera and species belonging to the Eurotiales (except those belonging to Elaphomycetaceae). The list includes 1 187 species, distributed over 27 genera, and contains MycoBank numbers, collection numbers of type and ex-type cultures, subgenus, section and series classification data, information on the mode of reproduction, and GenBank accession numbers of ITS, beta-tubulin (BenA), calmodulin (CaM) and RNA polymerase II second largest subunit (RPB2) gene sequences.

Článek

Purification and biochemical characterization of glucose-cellobiose-tolerant cellulases from Scytalidium thermophilum

Folia microbiologica. 2013 Nov ; 58 (6) : 561-8. [epub] 20130407

Folia Microbiol (Praha)
ISSN 1874-9356 | 0015-5632
Zdroj

Two cellulases from Scytalidium thermophilum were purified and characterized, exhibiting tolerance to glucose and cellobiose. Characterization of purified cellulases I and II by mass spectrometry revealed primary structure similarities with an exoglucanase and an endoglucanase, respectively. Molecular masses were 51.2 and 45.6 kDa for cellulases I and II, respectively, as determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis. Cellulases I and II exhibited isoelectric points of 6.2 and 6.9 and saccharide contents of 11 and 93 %, respectively. Optima of temperature and pH were 60-65 °C and 4.0 for purified cellulase I and 65 °C and 6.5 for purified cellulase II. Both cellulases maintained total CMCase activity after 60 min at 60 °C. Cysteine, Mn(2+), dithiotreitol and ß-mercaptoethanol-stimulated cellulases I and II. The tolerance to cellulose hydrolysis products and the high thermal stabilities of Scytalidium cellulases suggest good potential for industrial applications.

MeSH
aktivátory enzymů analýza MeSH
Ascomycota enzymologie MeSH
celobiosa metabolismus MeSH
celulasy chemie izolace a purifikace metabolismus MeSH
elektroforéza v polyakrylamidovém gelu MeSH
glukosa metabolismus MeSH
hmotnostní spektrometrie MeSH
inhibitory enzymů metabolismus MeSH
izoelektrický bod MeSH
koncentrace vodíkových iontů MeSH
molekulová hmotnost MeSH
stabilita enzymů MeSH
teplota MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
aktivátory enzymů MeSH
celobiosa MeSH
celulasy MeSH
glukosa MeSH
inhibitory enzymů MeSH

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