Fluorescein derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium. The highest decrease of absorbance A450 was observed in the growth phase regardless of the presence of inducers Tween 80 or poly(ethylene glycol) (PEG). Fluorescein linked to PEG was prepared and, after addition to cultures, shown to stimulate the production of lignin peroxidase. Passing of fluorescing substances into hyphae (observed by confocal microscopy) showed that they were concentrated on some structures inside hyphae.

• MeSH
• Enzyme Induction MeSH
• Fluorescein chemistry   metabolism   MeSH
• Fluorescent Dyes chemistry   metabolism   MeSH
• Hyphae metabolism   MeSH
• Culture Media MeSH
• Peroxidases metabolism   MeSH
• Phanerochaete growth & development   metabolism   MeSH
• Polyethylene Glycols chemistry   pharmacology   MeSH
• Polysorbates pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Fluorescein MeSH
• Fluorescent Dyes MeSH
• Culture Media MeSH
• lignin peroxidase MeSH Browser
• Peroxidases MeSH
• Polyethylene Glycols MeSH
• Polysorbates MeSH