Serotyping and some potential virulence-associated markers were investigated in Plesiomonas shigelloides strains isolated from humans, animals and aquatic environments. Surface properties of these strains were evaluated using Congo red binding, salt-aggregation test, bacterial adherence to xylene and motility. Production of pancreatic elastase, proteinase (consistent with subtilisin Carlsberg), triacylglycerol lipase, histidine decarboxylase and beta-hemolysin was also determined. In addition, detection of signal molecules such as C4-C8 unsubstituted N-acylhomoserine lactones (AHLs) was performed. The serological typing of the P. shigelloides strains showed that the isolates belonged to 13 different serovars. The majority of the strains were hydrophobic and motile. The strains produced low levels of elastase, proteinase and histidine decarboxylase whereas triacylglycerol lipase activity was relatively high. Only 23.3 % of the strains produced hemolysin. The AHLs signal molecules were not detected. P. shigelloides strains were able to produce a variety of potential virulence markers which may be involved in the pathogenesis of Plesiomonas-associated infections.

• MeSH
• bakteriální adheze MeSH
• druhová specificita MeSH
• gama-butyrolakton analogy a deriváty   metabolismus   MeSH
• gramnegativní bakteriální infekce mikrobiologie   MeSH
• hemolyziny biosyntéza   MeSH
• histidindekarboxylasa biosyntéza   MeSH
• lidé MeSH
• lipasa biosyntéza   MeSH
• mikrobiologie vody MeSH
• pankreatická elastasa biosyntéza   MeSH
• Plesiomonas klasifikace   izolace a purifikace   metabolismus   patogenita   MeSH
• sérotypizace MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• gama-butyrolakton MeSH
• hemolyziny MeSH
• histidindekarboxylasa MeSH
• homoserine lactone MeSH Prohlížeč
• lipasa MeSH
• pankreatická elastasa MeSH

The effect of aminoglycoside antibiotics (amikacin, gentamicin, netilmicin and tobramycin) at sublethal concentrations (sub-MICs) on some properties of Plesiomonas shigelloides strains was evaluated. All agents decreased the bacterial surface hydrophobicity. Amikacin (1/4 of the MIC) and netilmicin (1/4 and 1/8 of the MIC) changed the hydrophobic character of P. shigelloides surface to a hydrophilic one. Treatment of the strains with aminoglycosides decreased also motility, netilmicin being the most effective. No significant changes were found in lipolytic activity of antibiotic-treated strains. In the majority of cases aminoglycosides increased sensitivity of bacteria to hydrogen peroxide. The tested antibiotics did not induce production of short-chained N-acylhomoserine lactones signal molecules. Aminoglycosides at sub-MICs affected important activities of P. shigelloides potentially associated with their virulence in dependence on strain, antibiotic and concentration.

• MeSH
• amikacin farmakologie   MeSH
• antibakteriální látky farmakologie   MeSH
• gentamiciny farmakologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• mikrobiální testy citlivosti MeSH
• netilmicin farmakologie   MeSH
• oxidační stres MeSH
• Plesiomonas účinky léků   růst a vývoj   metabolismus   MeSH
• polysorbáty metabolismus   MeSH
• povrchově aktivní látky metabolismus   MeSH
• tobramycin farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• amikacin MeSH
• antibakteriální látky MeSH
• gentamiciny MeSH
• netilmicin MeSH
• polysorbáty MeSH
• povrchově aktivní látky MeSH
• tobramycin MeSH