Serotyping and some potential virulence-associated markers were investigated in Plesiomonas shigelloides strains isolated from humans, animals and aquatic environments. Surface properties of these strains were evaluated using Congo red binding, salt-aggregation test, bacterial adherence to xylene and motility. Production of pancreatic elastase, proteinase (consistent with subtilisin Carlsberg), triacylglycerol lipase, histidine decarboxylase and beta-hemolysin was also determined. In addition, detection of signal molecules such as C4-C8 unsubstituted N-acylhomoserine lactones (AHLs) was performed. The serological typing of the P. shigelloides strains showed that the isolates belonged to 13 different serovars. The majority of the strains were hydrophobic and motile. The strains produced low levels of elastase, proteinase and histidine decarboxylase whereas triacylglycerol lipase activity was relatively high. Only 23.3 % of the strains produced hemolysin. The AHLs signal molecules were not detected. P. shigelloides strains were able to produce a variety of potential virulence markers which may be involved in the pathogenesis of Plesiomonas-associated infections.

• MeSH
• Bacterial Adhesion MeSH
• Species Specificity MeSH
• 4-Butyrolactone analogs & derivatives   metabolism   MeSH
• Gram-Negative Bacterial Infections microbiology   MeSH
• Hemolysin Proteins biosynthesis   MeSH
• Histidine Decarboxylase biosynthesis   MeSH
• Humans MeSH
• Lipase biosynthesis   MeSH
• Water Microbiology MeSH
• Pancreatic Elastase biosynthesis   MeSH
• Plesiomonas classification   isolation & purification   metabolism   pathogenicity   MeSH
• Serotyping MeSH
• Virulence MeSH
• Animals MeSH
• Check Tag
• Humans MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• 4-Butyrolactone MeSH
• Hemolysin Proteins MeSH
• Histidine Decarboxylase MeSH
• homoserine lactone MeSH Browser
• Lipase MeSH
• Pancreatic Elastase MeSH

The effect of aminoglycoside antibiotics (amikacin, gentamicin, netilmicin and tobramycin) at sublethal concentrations (sub-MICs) on some properties of Plesiomonas shigelloides strains was evaluated. All agents decreased the bacterial surface hydrophobicity. Amikacin (1/4 of the MIC) and netilmicin (1/4 and 1/8 of the MIC) changed the hydrophobic character of P. shigelloides surface to a hydrophilic one. Treatment of the strains with aminoglycosides decreased also motility, netilmicin being the most effective. No significant changes were found in lipolytic activity of antibiotic-treated strains. In the majority of cases aminoglycosides increased sensitivity of bacteria to hydrogen peroxide. The tested antibiotics did not induce production of short-chained N-acylhomoserine lactones signal molecules. Aminoglycosides at sub-MICs affected important activities of P. shigelloides potentially associated with their virulence in dependence on strain, antibiotic and concentration.

• MeSH
• Amikacin pharmacology   MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Gentamicins pharmacology   MeSH
• Hydrophobic and Hydrophilic Interactions MeSH
• Microbial Sensitivity Tests MeSH
• Netilmicin pharmacology   MeSH
• Oxidative Stress MeSH
• Plesiomonas drug effects   growth & development   metabolism   MeSH
• Polysorbates metabolism   MeSH
• Surface-Active Agents metabolism   MeSH
• Tobramycin pharmacology   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Amikacin MeSH
• Anti-Bacterial Agents MeSH
• Gentamicins MeSH
• Netilmicin MeSH
• Polysorbates MeSH
• Surface-Active Agents MeSH
• Tobramycin MeSH