Calcofluor-allied optical brightener Rylux BSU stimulated spore germination rate in Trichophyton mentagrophytes and Aspergillus fumigatus both if supplemented into Sabouraud glucose agar and if used for pretreatment of spore suspension prior to inoculation at low concentrations. Maximum stimulation of germination was obtained if 0.2% Rylux BSU was used for pretreatment in aqueous solution for 1 d prior to inoculation (130% in T. mentagrophytes and 150% in A. fumigatus, respectively). Pretreatment with 1% Rylux BSU provided strong protection against UV-irradiation and resulted in increased yields of cultural variants after UV-irradiation.

• MeSH
• Aspergillus fumigatus drug effects   growth & development   metabolism   radiation effects   MeSH
• Benzenesulfonates pharmacology   MeSH
• Fluorescent Dyes MeSH
• Culture Media MeSH
• Microscopy, Electron, Scanning MeSH
• Spores, Fungal growth & development   MeSH
• Trichophyton drug effects   growth & development   metabolism   radiation effects   MeSH
• Ultraviolet Rays MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Benzenesulfonates MeSH
• Fluorescent Dyes MeSH
• Culture Media MeSH
• Rylux BSU MeSH Browser

Transcription from the chiC promoter, directing expression of the chitinase gene, chiC, in Streptomyces coelicolor, was analyzed using xylE reporter gene and high-resolution S1-nuclease mapping. The transcription from the chiC promoter was induced by chitin, and this induction was dramatically reduced in the S. coelicolor chiR-disrupted strain. This indicated a dependence of chiC expression upon the chiR gene encoding a response regulator protein. To investigate this relationship, the S. coelicolor ChiR was overproduced using Escherichia coli T7 RNA polymerase expression system. However, gel mobility shift-assay with such a purified ChiR showed no binding in the chiC promoter region, which indicates a lack of specific phosphorylation of E. coli overproduced ChiR that is necessary for DNA-binding activity of response regulators.

• MeSH
• Bacterial Proteins genetics   metabolism   MeSH
• Chitinases genetics   metabolism   MeSH
• Single-Strand Specific DNA and RNA Endonucleases MeSH
• Escherichia coli enzymology   genetics   MeSH
• Transcription, Genetic * MeSH
• Molecular Sequence Data MeSH
• Promoter Regions, Genetic MeSH
• Gene Expression Regulation, Bacterial * MeSH
• Restriction Mapping MeSH
• Amino Acid Sequence MeSH
• Base Sequence MeSH
• Streptomyces enzymology   genetics   MeSH
• Transcription Factors * MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Bacterial Proteins MeSH
• ChiR protein, Streptomyces MeSH Browser
• chitinase C-1 MeSH Browser
• Chitinases MeSH
• Single-Strand Specific DNA and RNA Endonucleases MeSH
• Transcription Factors * MeSH