The occurrence of GC clusters in Saccharomyces spp. and related yeasts was examined to clarify their association with the stability of intact mitochondrial genome. Abundance of nonspecific or specific GC clusters in these species decreases with phylogenetic distance from S. cerevisiae. Their number but not the number of replication origins correlates with the ability to form respiration-deficient mutants induced by ethidium bromide. This effect is not associated with the nuclear background since the cybrids having identical nuclei and mitochondria from different species gave similar results. In contrast to grand genomes, the presence of GC clusters in rho- mutants does not play any role in ethidium bromide induced mtDNA loss. The most plausible explanation for mitotically lost petite mtDNA seems to be dilution during the distribution.

• MeSH
• DNA fungální chemie   účinky léků   MeSH
• ethidium farmakologie   MeSH
• GC bohatá sekvence * účinky léků   MeSH
• genotyp MeSH
• mitochondriální DNA chemie   MeSH
• mutace MeSH
• restrikční mapování MeSH
• Saccharomyces cerevisiae účinky léků   genetika   MeSH
• zastoupení bazí MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA fungální MeSH
• ethidium MeSH
• mitochondriální DNA MeSH