4-Nitrophenyl alpha-D-galactopyranosyl-(1-->3)-6-O-acetyl-alpha-D-galactopyranoside was prepared in a transglycosylation reaction catalyzed by alpha-D-galactosidase from Talaromyces flavus using 4-nitrophenyl alpha-D-galactopyranoside as a glycosyl donor and 4-nitrophenyl 6-O-acetyl-alpha-D-galactopyranoside as an acceptor. 4-Nitrophenyl 6-O-acetyl-alpha-D-galactopyranoside and 4-nitrophenyl 6-O-acetyl-beta-D-galactopyranoside were prepared in a regioselective enzymic transesterification in pyridine-acetone catalyzed by the lipase PS from Burkholderia cepacia. A series of water-miscible organic solvents (acetone, acetonitrile, dimethylformamide, dimethyl sulfoxide, 1,4-dioxane, 2-methoxyethanol, pyridine, 2-methylpropan-2-ol, tetrahydrofuran, propargyl alcohol) were used as co-solvents in this enzymic reaction. Their influence on the activity and stability of the alpha-galactosidase from T. flavus was established. 2-Methylpropan-2-ol and acetone (increasing the solubility of the modified substrate acceptors and displaying the minimum impairment of the activity and stability of the enzyme) were used as co-solvents in transglycosylation reactions.

• MeSH
• alfa-galaktosidasa chemie   metabolismus   MeSH
• Burkholderia cepacia enzymologie   MeSH
• disacharidy biosyntéza   chemická syntéza   MeSH
• katalýza MeSH
• konformace sacharidů MeSH
• magnetická rezonanční spektroskopie MeSH
• molekulární struktura MeSH
• nitrofenylgalaktosidy biosyntéza   chemická syntéza   MeSH
• rozpouštědla MeSH
• sacharidové sekvence MeSH
• Talaromyces enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• alfa-galaktosidasa MeSH
• disacharidy MeSH
• nitrofenylgalaktosidy MeSH
• rozpouštědla MeSH

A total of 307 new compounds, natural, semisynthetic or synthetic, were isolated at the Institute of microbiology during the last twelve years. Due to the development of separation (chromatographic) methods and of analytical methods used to determine the chemical structure of these compounds, i.e. NMR, MS and X-ray diffraction, many new metabolites could be described.

• MeSH
• biologické faktory chemie   izolace a purifikace   MeSH
• cyklické peptidy chemie   izolace a purifikace   MeSH
• enzymy chemie   izolace a purifikace   MeSH
• molekulární sekvence - údaje MeSH
• námelové alkaloidy chemie   izolace a purifikace   MeSH
• sacharidové sekvence MeSH
• sacharidy chemie   izolace a purifikace   MeSH
• sekvence aminokyselin MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• přehledy MeSH
• Názvy látek
• biologické faktory MeSH
• cyklické peptidy MeSH
• enzymy MeSH
• námelové alkaloidy MeSH
• sacharidy MeSH