Thirty-three isolates belonging to six species of the genus Trichoderma were tested for the ability to hydroxylate progesterone to 11alpha-, 11beta-, 11alpha,17alpha- and 6beta, 17alpha-derivatives, and epicortisol. T. aureoviride, T. harzianum, T. polysporum and T. pseudokoningii produced 11alpha-hydroxyprogesterone. T. harzianum and T. hamatum can form only the 11beta-isomer. T. koningii and T. hamatum produced 11alpha-, 11beta-, 11alpha,17alpha- and 6beta,11alpha-hydroxy derivatives. 11alpha, 11beta, 6beta,11alpha- and 11alpha,17alpha-hydroxyprogesterones and epicortisol are produced by T. aureoviride and T. pseudokoningii. Cortisol was produced only when the medium was fortified by 10 g/L peptone. This is the first record of conversion of progesterone to mono-, di- and trihydroxyprogesterones by these Trichoderma species.

• MeSH
• Biotransformation MeSH
• Hydroxylation MeSH
• Progesterone chemistry   metabolism   MeSH
• Trichoderma growth & development   isolation & purification   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Progesterone MeSH

Yeast strains Cryptococcus albidus, Cryptococcus laurentii, Rhodotorula minuta were isolated from a moss sample. Candida oleophila and Rhodotorula mucilaginosa were isolated from a soil sample taken from Livingston Island. Antarctica. Fatty acid, phospholipid, sterol and tocopherol composition was determined in separated lipid fraction after fermentation in a medium containing glucose, peptone and yeast extract. Unsaturated fatty acids, mainly oleic (51-65%) and linoleic (9.5-16.8%), predominated in triacylglycerols. Sterols represent ca. 120-930 mg per kg dry biomass. The content of major phospholipids (phosphatidylcholine, phosphatidylinositol and phosphatidylethanolamine) was ca. 100-800 mg/kg. The amount of tocopherols (mainly gamma- and delta-tocopherol) was 2.1-6.3 mg/kg.

• MeSH
• Phospholipids analysis   MeSH
• Yeasts chemistry   MeSH
• Lipids analysis   chemistry   MeSH
• Fatty Acids analysis   MeSH
• Soil Microbiology MeSH
• Sterols analysis   MeSH
• Tocopherols analysis   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Geographicals
• Antarctic Regions MeSH
• Names of Substances
• Phospholipids MeSH
• Lipids MeSH
• Fatty Acids MeSH
• Sterols MeSH
• Tocopherols MeSH

A strain of Micrococcus sp. was isolated for its capability of side chain degradation of cholesterol. This strain was characterized and identified as Micrococcus roseus. It was found to be the best strain for the production of androsta-1,4-diene-3,17-dione and androst-4-ene-3,17-dione compared with other Micrococcus strains.

• MeSH
• Androstadienes metabolism   MeSH
• Androstenedione metabolism   MeSH
• Biotransformation MeSH
• Cholesterol metabolism   MeSH
• Rats MeSH
• Fatty Acids analysis   MeSH
• Micrococcus classification   genetics   isolation & purification   metabolism   MeSH
• Plasmids genetics   MeSH
• Animals MeSH
• Check Tag
• Rats MeSH
• Animals MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• 1,4-androstadiene-3,17-dione MeSH Browser
• Androstadienes MeSH
• Androstenedione MeSH
• Cholesterol MeSH
• Fatty Acids MeSH