The minimum inhibitory concentrations (MICs) of 24 antibiotics were determined for 45 Stenotrophomonas maltophilia strains by the microdilution method at 37 and 30 degrees C (after 24 h and 48 h of incubation). The isolates were obtained from mouth swabs and pus of 116 captive snakes whereas the identical strains (based on PFGE) of the same origin were discarded. At 37 degrees C, the isolates showed a low frequency of resistance to levofloxacin (0 and 8.9 % of resistant strains after 24 and 48 h, MICs(50) 0.5 and 1 mg/L, MICs(90) 1 and 2 mg/L) and cotrimoxazole (2.2 % of resistant strains for 24 and 48 h, MICs(50) 4 mg/L for both time periods, MICs(90) 4 and 8). At 30 degrees C, the most effective drugs were also cotrimoxazole (2.2 and 6.7 %, MICs(50) 4 and 8, MICs(90) 8 and 32) and levofloxacin (8.9 and 46.7 %, MICs(50) 1 and 2, MICs(90) 2 and 4). The isolates were either identically or more susceptible to antibiotics than strains acquired from patients hospitalized at Olomouc University Hospital (the same region) with the exception of ciprofloxacin, cefoperazone, cefoperazone/sulbactam and ceftazidime.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence * MeSH
• gramnegativní bakteriální infekce mikrobiologie   veterinární   MeSH
• hadi mikrobiologie   MeSH
• hnisání mikrobiologie   MeSH
• mikrobiální testy citlivosti MeSH
• přenašečství mikrobiologie   veterinární   MeSH
• Stenotrophomonas maltophilia účinky léků   izolace a purifikace   MeSH
• ústa mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antibakteriální látky MeSH

Isolates of Stenotrophomonas maltophilia species display the feature "temperature-dependent susceptibility" (TDS) to antibiotics. Both 30TDS strains (at least 4 times lower value of minimum inhibitory concentration (MIC) of an antibiotic at 30 than at 37 degrees C) and 37TDS strains (at least 4 times lower value of MIC at 37 than at 30 degrees C) were described. Changes in the distribution of saturated and unsaturated fatty acids (FA) at 30 and 37 degrees C were considered as one of possible causes of the TDS phenomenon. Gas chromatography was used to determine the distribution of individual FA in five 37TDS strains of S. maltophilia (Group I); in five strains with MIC values unaffected by the cultivation temperature (Group II) and in six 30TDS (four strains) or 30/37TDS (two strains) isolates (Group III). At identical temperatures, no statistically significant differences in the distribution of major FA (iso-15:0, anteiso-15:0, 16:0 and 16:1) were registered between individual groups. Statistically significant (p < 0.05) differences between groups were found in minor FA only (iso-16:0, iso-17:0 and iso-17:1). Distribution changes of cellular FA at 30 and 37 degrees C can be considered to play only a minor role in the formation of the TDS phenomenon.

• MeSH
• antibakteriální látky farmakologie   MeSH
• kyseliny mastné neesterifikované metabolismus   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• Stenotrophomonas maltophilia účinky léků   metabolismus   MeSH
• teplota MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• kyseliny mastné neesterifikované MeSH