The GATC-specific restriction and modification activities were analyzed in 11 major bacterial representatives of ruminal microflora. Modification phenotype was observed in 13 out of 40 ruminal strains. MboI isoschizomeric restriction endonucleases were detected in 10 bacterial strains tested; three strains lacked any detectable corresponding endonuclease activity. The only examined strain of Mitsuokella multi-acida was found to possess a different type of endonuclease activity. This is the first report on restriction activity in ruminal treponemes M. multiacida and Megasphaera elsdenii.

• MeSH
• bachor mikrobiologie   MeSH
• Bacteria enzymologie   izolace a purifikace   MeSH
• DNA restrikčně-modifikační enzymy metabolismus   MeSH
• DNA virů metabolismus   MeSH
• elektroforéza v agarovém gelu MeSH
• Megasphaera enzymologie   izolace a purifikace   MeSH
• restrikční endonukleasy typu II metabolismus   MeSH
• substrátová specifita MeSH
• Veillonellaceae enzymologie   izolace a purifikace   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA restrikčně-modifikační enzymy MeSH
• DNA virů MeSH
• GATC-specific type II deoxyribonucleases MeSH Prohlížeč
• restrikční endonukleasy typu II MeSH

A high frequency of plasmids was detected in the rumen bacterium Selenomonas ruminantium. Plasmids 0.9-20 kb in size were detected in more than 50% tested strains. Densitometric analysis indicated that plasmid DNA could represents more than 25% of total cellular DNA. Up to six plasmids were detected in strain S. ruminantium 18. Two smallest cryptic plasmids pSRD181 and pSRD182 from this strain were cloned into Escherichia coli vector pBluescriptSK+ and partially characterized. The plasmid pSRD181 is 1.4 kb and pSRD182 is 2.0 kb. While computer analysis of pSRD181 sequence data showed high homology with replication protein of Staphylococcus aureus plasmids, the pSRD182 sequence showed no significant homology in GenBank data. Strain S. ruminantium 28 was successfully transformed with pJW1 derived plasmid pJ1B1 using ampicillin resistance gene as marker. This is the first report on transformation of selenomonads with foreign DNA.

• MeSH
• bachor mikrobiologie   MeSH
• bakteriální transformace * MeSH
• DNA bakterií genetika   MeSH
• Escherichia coli genetika   MeSH
• genetické vektory * MeSH
• klonování DNA MeSH
• plazmidy genetika   MeSH
• rezistence na ampicilin genetika   MeSH
• Selenomonas genetika   růst a vývoj   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA bakterií MeSH