(1) Background: Microorganisms originating from the microflora of the oral cavity are the main cause of the inflammatory diseases of the dental pulp and periapical periodontium, as well as the failure of endodontic treatment. The subsequent root canal treatment is not able to remove all the pathogens, and a small number of viable bacteria remain in the dentine tubules, which must be sealed by endodontic sealers. These sealers should have at least a bacteriostatic effect to prevent the remaining bacteria from reproducing. The aim of this study is to compare the short-term antibacterial activity of three endodontic sealers based on poly-epoxy resin, zinc oxide-eugenol and calcium silicate with a calcium hydroxide-based sealer. Calcium hydroxide is used as temporary intracanal medicament and, thus, should show significant antibacterial activity. (2) Methods: A total of 25 bovine dentine samples infected with Enterococcus faecalis were used in this study. After the sealer placement and a 24 h incubation period, the root canal walls were scraped, and the suspension of dentine fillings was used for a semi-quantitative evaluation of microbial growth. (3) Results: The poly-epoxide resin-based sealer ADSeal™ showed significant antibacterial properties. (4) Conclusions: The highest antibacterial activity was shown in poly-epoxide resin-based sealer group, followed by the zinc oxide-eugenol-based sealer and calcium silicate-based sealer.

• Klíčová slova
• antibacterial, antimicrobial, calcium silicate, endodontic, poly-epoxy resin, sealer, zinc oxide-eugenol,
• Publikační typ
• časopisecké články MeSH

Three root canal filling materials, viz. calcium hydroxide-based cement (Apexit, resin-based cement (AH-plus) and glass-ionomer based material (Ketac Endo) were tested for their influence on several functions of peritoneal macrophages from Balb/c mice. Macrophage functions were evaluated by the adherence, phagocytic, candidacidal and Nitro blue tetrazolium-dye assays. Ketac-Endo enhanced all macrophage functions in the first 2 d (p < or = 0.05), when compared to the positive control, but this effect had changed after 7 and 14 d, causing inhibition of these functions. Other materials suppressed substrate adherence capacity and phagocytosis, while significantly stimulating macrophage microbicidal activity (p < or = 0.05) in a time-dependent manner.

• MeSH
• buněčná adheze účinky léků   MeSH
• Candida albicans účinky léků   růst a vývoj   MeSH
• epoxidové pryskyřice farmakologie   MeSH
• fagocytóza účinky léků   MeSH
• hydroxid vápenatý farmakologie   MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• peritoneální makrofágy účinky léků   imunologie   fyziologie   MeSH
• skloionomerní cementy farmakologie   MeSH
• tetrazoliová modř metabolismus   MeSH
• výplňové materiály kořenových kanálků klasifikace   farmakologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• Apexit MeSH Prohlížeč
• epoxidové pryskyřice MeSH
• epoxy resin-based root canal sealer MeSH Prohlížeč
• hydroxid vápenatý MeSH
• Ketac Endo MeSH Prohlížeč
• skloionomerní cementy MeSH
• tetrazoliová modř MeSH
• výplňové materiály kořenových kanálků MeSH