Streptomyces caelestis DSM 40084 produces two osmolytes, viz. 2-O-(alpha-D-glucopyranosyl)-zeta-glyceric acid (GG) and trehalose. Both compounds were isolated and identified by nuclear magnetic resonance spectroscopy and mass spectrometry. A very sensitive regulation of the cell osmolytes was demonstrated in exponentially growing cultures. The intracellular levels of GG and trehalose increased 2x in response to a step change of medium osmolarity caused by 0.3% NaCl. 1H NMR analysis of the cell extracts did not confirm the presence of additional osmolytes. GG is a S. caelestis metabolite commonly released from the cells; its concentration reached 3 g/L during the cultivation in a yeast extract--(NH4)2SO4-glycerol medium. This is the first report on the occurrence of the ionic osmolyte GG in the genus Streptomyces and on its free excretion to the medium.

• MeSH
• Disaccharides isolation & purification   MeSH
• Extracellular Space chemistry   metabolism   MeSH
• Glycosylation MeSH
• Culture Media chemistry   MeSH
• Glyceric Acids agonists   chemistry   isolation & purification   metabolism   MeSH
• Magnetic Resonance Spectroscopy MeSH
• Osmolar Concentration MeSH
• Osmosis MeSH
• Streptomyces chemistry   metabolism   MeSH
• Trehalose chemistry   isolation & purification   metabolism   MeSH
• Water-Electrolyte Balance * MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Disaccharides MeSH
• glyceric acid MeSH Browser
• Culture Media MeSH
• Glyceric Acids MeSH
• Trehalose MeSH

A total of 307 new compounds, natural, semisynthetic or synthetic, were isolated at the Institute of microbiology during the last twelve years. Due to the development of separation (chromatographic) methods and of analytical methods used to determine the chemical structure of these compounds, i.e. NMR, MS and X-ray diffraction, many new metabolites could be described.

• MeSH
• Biological Factors chemistry   isolation & purification   MeSH
• Peptides, Cyclic chemistry   isolation & purification   MeSH
• Enzymes chemistry   isolation & purification   MeSH
• Molecular Sequence Data MeSH
• Ergot Alkaloids chemistry   isolation & purification   MeSH
• Carbohydrate Sequence MeSH
• Carbohydrates chemistry   isolation & purification   MeSH
• Amino Acid Sequence MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH
• Names of Substances
• Biological Factors MeSH
• Peptides, Cyclic MeSH
• Enzymes MeSH
• Ergot Alkaloids MeSH
• Carbohydrates MeSH