Yarrowia lipolytica plasma-membrane Na+/H+ antiporter, encoded by the YlNHA2 gene, is a very efficient exporter of surplus sodium from the cytosol. Its heterologous expression in Saccharomyces cerevisiae wild-type laboratory strains increased their sodium tolerance more efficiently than the expression of ZrSod2-22 antiporter from the osmotolerant yeast Zygosaccharomvces rouxii.

• MeSH
• Antifungal Agents pharmacology   MeSH
• Gene Expression MeSH
• Fungal Proteins genetics   metabolism   MeSH
• Cloning, Molecular MeSH
• Sodium-Hydrogen Exchangers genetics   metabolism   MeSH
• Recombinant Proteins genetics   metabolism   MeSH
• Saccharomyces cerevisiae drug effects   genetics   growth & development   metabolism   MeSH
• Salts pharmacology   MeSH
• Yarrowia enzymology   genetics   MeSH
• Zygosaccharomyces enzymology   genetics   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Antifungal Agents MeSH
• Fungal Proteins MeSH
• Sodium-Hydrogen Exchangers MeSH
• Recombinant Proteins MeSH
• Salts MeSH

The Saccharomyces cerevisiae genome contains three genes encoding alkali metal cation/H+ antiporters (Nha1p, Nhx1p, Kha1p) that differ in cell localization, substrate specificity and physiological function. Systematic genome sequencing of other yeast species revealed highly conserved homologous ORFs in all of them. We compared the yeast sequences both at DNA and protein levels. The subfamily of yeast endosomal/prevacuolar Nhx1 antiporters is closely related to mammalian plasma membrane NHE proteins and to both plasma membrane and vacuolar plant antiporters. The high sequence conservation within this subfamily of yeast antiporters suggests that Nhx1p is of great importance in cell physiology. Yeast Kha1 proteins probably belong to the same subfamily as bacterial antiporters, whereas Nhal proteins form a distinct subfamily.

• MeSH
• DNA, Fungal analysis   MeSH
• Potassium-Hydrogen Antiporters chemistry   classification   genetics   MeSH
• Phylogeny MeSH
• Membrane Proteins chemistry   classification   genetics   MeSH
• Molecular Sequence Data MeSH
• Sodium-Hydrogen Exchangers chemistry   classification   genetics   MeSH
• Cation Transport Proteins chemistry   classification   genetics   MeSH
• Saccharomyces cerevisiae Proteins chemistry   classification   genetics   MeSH
• Amino Acid Sequence MeSH
• Sequence Analysis, DNA MeSH
• Sequence Homology, Amino Acid MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• DNA, Fungal MeSH
• Potassium-Hydrogen Antiporters MeSH
• KHA1 protein, S cerevisiae MeSH Browser
• Membrane Proteins MeSH
• Sodium-Hydrogen Exchangers MeSH
• NHA1 protein, S cerevisiae MeSH Browser
• NHX1 protein, S cerevisiae MeSH Browser
• Cation Transport Proteins MeSH
• Saccharomyces cerevisiae Proteins MeSH

A triple mutant strain of Saccharomyces cerevisiae lacking its own Na+-ATPases and Na+/H+ antiporters (enal-4delta nha1delta nhx1delta) was used for the expression of the Oryza sativa NHX1 gene encoding a putative vacuolar Na+/H+ exchanger. Upon expression in yeast cells, the OsNhx 1p is not a transport system specific only for sodium cations but it has a broad substrate specificity for at least four alkali metal cations (Na+, Li+, K+ and Rb+) and is able to substitute for the endogenous yeast ScNhx1 antiporter. Its activity contributes to sequestration of alkali metal cations in intracellular vesicles.

• MeSH
• DNA, Plant genetics   MeSH
• Genes, Fungal MeSH
• Metals metabolism   pharmacology   MeSH
• Mutation MeSH
• Sodium-Hydrogen Exchangers genetics   metabolism   MeSH
• Recombinant Proteins genetics   metabolism   MeSH
• Genes, Plant MeSH
• Oryza genetics   metabolism   MeSH
• Saccharomyces cerevisiae Proteins genetics   metabolism   MeSH
• Saccharomyces cerevisiae drug effects   genetics   metabolism   MeSH
• Base Sequence MeSH
• Genetic Complementation Test MeSH
• Vacuoles metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• DNA, Plant MeSH
• Metals MeSH
• Sodium-Hydrogen Exchangers MeSH
• Recombinant Proteins MeSH
• Saccharomyces cerevisiae Proteins MeSH