Resistance to 17 antimicrobials, surface hydrophobicity, motility, biofilm, production of N-acylhomoserine lactone signal molecules (N-butyrylhomoserine lactone and N-3-oxolauroylhomoserine lactone) and response to oxidative stress were analyzed in 47 clinical Pseudomonas aeruginosa strains. In addition to natural resistance, the strains demonstrated the greatest level of resistance to cefotaxime (91.5%). Isolates in the range of 44.7-57.4% were resistant to aminoglycosides and ciprofloxacin, of 25.5-36.2% to cephalosporins. On the other hand, 97.9% remained susceptible to meropenem, 93.6% to piperacillin + tazobactam and 87.2% to piperacillin. The majority of the strains (72.3%) manifested their hydrophilic character. Higher zones of motility showed 12 isolates (in average 54.8 mm) as compared to the others (30.2 mm). Approximately 1/3 of the strains (29.8%) produced a higher amount of biofilm quantified by measuring the absorbance of solubilized crystal violet (0.20-0.46) than the rest of isolates (0-0.19). All but two strains produced N-3-oxolauroylhomoserine lactone and in 48.9% of samples N-butyrylhomoserine lactone were detected. Only four isolates with higher biofilm production showed both types of homoserine lactone. Majority of the strains (70.2%) manifested higher resistance to H2O2 than the rest of the strains. The group of strains resistant to aminoglycosides and ciprofloxacin revealed a significantly higher number of hydrophobic strains (compared with the sensitive ones). In contrast, higher number of strains sensitive to aminoglycosides and ciprofloxacin or only to ciprofloxacin produced N-butyrylhomoserine lactone and biofilm (compared to the resistant ones). Such association was not found among the rest of the tested parameters. The results indicate that the resistance to antimicrobials in P. aeruginosa isolates was not generally associated with changes in the production of the pathogenicity factors.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence * MeSH
• biofilmy růst a vývoj   MeSH
• fenotyp MeSH
• fyziologická adaptace MeSH
• gama-butyrolakton analogy a deriváty   biosyntéza   MeSH
• genciánová violeť metabolismus   MeSH
• hydrofobní a hydrofilní interakce MeSH
• lidé MeSH
• oxidační stres MeSH
• oxidancia farmakologie   MeSH
• peroxid vodíku farmakologie   MeSH
• pohyb MeSH
• pseudomonádové infekce mikrobiologie   MeSH
• Pseudomonas aeruginosa účinky léků   izolace a purifikace   patogenita   MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• gama-butyrolakton MeSH
• genciánová violeť MeSH
• oxidancia MeSH
• peroxid vodíku MeSH

Effect of pH on production of extracellular virulence factors of Pseudomonas aeruginosa grown on catheter in biofilm was determined. Alginate and proteinase production was higher at pH 8; in contrast, siderophores (pyochelin and pyoverdin) were synthesized more intensively at pH 5.

• MeSH
• algináty metabolismus   MeSH
• bakteriální proteiny metabolismus   MeSH
• biofilmy růst a vývoj   MeSH
• faktory virulence biosyntéza   MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média speciální chemie   MeSH
• kyselina glukuronová metabolismus   MeSH
• kyseliny hexuronové metabolismus   MeSH
• lidé MeSH
• moč mikrobiologie   MeSH
• proteasy metabolismus   MeSH
• pseudomonádové infekce mikrobiologie   MeSH
• Pseudomonas aeruginosa růst a vývoj   metabolismus   patogenita   fyziologie   MeSH
• siderofory metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• algináty MeSH
• bakteriální proteiny MeSH
• faktory virulence MeSH
• kultivační média speciální MeSH
• kyselina glukuronová MeSH
• kyseliny hexuronové MeSH
• proteasy MeSH
• siderofory MeSH

A total of 5 Bifidobacterium spp. isolated from pig and children' feces and 6 Lactobacillus spp. from chicken feces were examined for expression of aggregation, cell surface hydrophobicity (CSH) and adherence to intestinal mucin. Co-aggregation activity was seen in 3 strains of auto-aggregative bifidobacteria and 4 auto-aggregative strains of Lactobacillus spp. with 2 enterohemorrhagic E. coli (O157). CSH correlated with Lactobacillus auto-aggregating activity and adherence to mucin but the correlation between Bifidobacterium adherence to mucin and CSH was not confirmed.

• MeSH
• bakteriální adheze * MeSH
• Bifidobacterium izolace a purifikace   fyziologie   MeSH
• Escherichia coli O157 fyziologie   MeSH
• feces mikrobiologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• kur domácí mikrobiologie   MeSH
• Lactobacillus izolace a purifikace   fyziologie   MeSH
• lidé MeSH
• muciny metabolismus   MeSH
• prasata mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• muciny MeSH

Planktonic and biofilm cells of a clinical urinary isolate of P. aeruginosa were compared in vitro for their ability to adhere to uroepithelial cells, interaction with macrophages, and for production of virulence factors like extracellular proteinase, elastase, hemolysin, phospholipase C and pyochelin. Biofilm cells showed increased adherence to UECs, which was coupled with reduced uptake and intracellular killing by macrophages. Overall there was a decrease in production of extracellular products by biofilm cells. Comparing the two cell forms for their ability to establish infection in an ascending model of acute pyelonephritis, significant enhancement of renal bacterial load, as well as more pronounced renal pathology developed with biofilm cells.

• MeSH
• biofilmy * MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• plankton patogenita   MeSH
• Pseudomonas aeruginosa patogenita   MeSH
• pyelonefritida etiologie   MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

The role of Klebsiella pneumoniae K- and O-polysaccharide antigens was determined in a rat model of lobar pneumonia. The induction of experimental infection in rats by wild-type strain, and its lipopolysaccharide- and capsular polysaccharide-deficient mutants was compared. Though the mutant lacking both antigens (K- O-) induced infection it could not successfully establish itself in the rat lung. It caused only mild infection, as compared to the wild type strain (K+ O+) and the strain lacking CPS alone (K- O+). Besides capsular polysaccharide, the lipopolysaccharide antigen was shown to be an important factor in pathogenesis of K. pneumoniae acute respiratory tract infection.

• MeSH
• antigeny bakteriální metabolismus   MeSH
• bakteriální pouzdra metabolismus   MeSH
• infekce bakteriemi rodu Klebsiella mikrobiologie   patologie   MeSH
• infekce dýchací soustavy mikrobiologie   patologie   MeSH
• Klebsiella pneumoniae metabolismus   patogenita   MeSH
• krysa rodu Rattus MeSH
• lipopolysacharidy metabolismus   MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antigeny bakteriální MeSH
• lipopolysacharidy MeSH

An isolate of Pseudomonas aeruginosa from cystic fibrosis was highly resistant to beta-lactams and beta-lactamase inhibitors. The resistant determinants of clinical isolate to imipenem, ceftazidim, cefriaxone and cefepime were conjugally nontransferable. The slow or nonenzymically mediated breakdown of imipenem and other broad-spectrum beta-lactams suggested the resistance of P. aeruginosa isolate to these drugs which may be attributed to both permeability and efflux. Impaired penetration of imipenem and other beta-lactams through the membrane was detected by a diminished expression of outer-membrane proteins of approximate molar mass of 46 and 39 kDa, matched to OprD and OprF, respectively. Efflux resistance mechanism for meropenem and beta-lactams has been ruled out since the isolate failed to express outer-membrane protein of approximately 50 kDa which is matched to the OprM protein channel. Thus, reduced permeability in the clinical isolate is the main mechanism conferring resistance against beta-lactams including imipenem.

• MeSH
• aktivní transport MeSH
• antibakteriální látky metabolismus   farmakologie   MeSH
• bakteriální geny MeSH
• cefalosporiny metabolismus   MeSH
• cefepim MeSH
• ceftazidim metabolismus   farmakologie   MeSH
• ceftriaxon metabolismus   farmakologie   MeSH
• cystická fibróza mikrobiologie   MeSH
• elektroforéza v polyakrylamidovém gelu MeSH
• imipenem metabolismus   farmakologie   MeSH
• konjugace genetická MeSH
• lidé MeSH
• membránové transportní proteiny metabolismus   MeSH
• mikrobiální testy citlivosti MeSH
• mnohočetná bakteriální léková rezistence genetika   MeSH
• permeabilita buněčné membrány MeSH
• poriny metabolismus   MeSH
• proteiny vnější bakteriální membrány metabolismus   MeSH
• Pseudomonas aeruginosa účinky léků   genetika   metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• cefalosporiny MeSH
• cefepim MeSH
• ceftazidim MeSH
• ceftriaxon MeSH
• imipenem MeSH
• membránové transportní proteiny MeSH
• OprD protein, Pseudomonas aeruginosa MeSH Prohlížeč
• OprM protein, Pseudomonas aeruginosa MeSH Prohlížeč
• poriny MeSH
• proteiny vnější bakteriální membrány MeSH