Lactococcus lactis subsp. lactis has been isolated from the intestines of marine fish and is a candidate probiotic for aquaculture. In order to use the bacterium as a probiotic, properties such as bile tolerance need to be assessed. Here, we compared bile tolerance in L. lactis strains derived from different sources. Three L. lactis subsp. lactis strains from marine fish (MFL), freshwater fish (FFL), and cheese starter (CSL) were used along with an Lactococcus lactis subsp. cremoris strain from cheese starter (CSC). The four strains were grown under various culture conditions: deMan-Rogosa-Sharpe (MRS) broth containing bile salts/acids, MRS agar containing oxgall, and phosphate-buffered saline (PBS) containing fish bile. Survival/growth of the strains in the presence of sodium cholate and sodium deoxycholate varied in the order MFL, CSL > CSC > FFL; in the presence of sodium taurocholate, the order was MFL > CSL > CSC > FFL. In liquid media containing various concentrations of oxgall, survival of the strains was observed in the order MFL > CSL > FFL and CSC. The survival of MFL was not affected by bile collected from the goldfish (Carassius auratus subsp. auratus) or the puffer fish (Takifugu niphobles), although the other strains showed significant inhibition of growth. It is a novel and beneficial finding that MFL has the highest resistance to bile acid.

• MeSH
• Lactococcus lactis metabolismus   MeSH
• počet mikrobiálních kolonií MeSH
• probiotika metabolismus   terapeutické užití   MeSH
• ryby mikrobiologie   MeSH
• vodní hospodářství MeSH
• žlučové kyseliny a soli farmakologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• žlučové kyseliny a soli MeSH

The activity of antagonistic substances produced by Pseudomonas aeruginosa and Lactobacillus acidophilus against the planktonic and sessile populations of Staphylococcus aureus strains was demonstrated. The strongest effects were caused by probiotic L. acidophilus strain - bacteriocin-like inhibitory substances (BLIS) positive. However, the S. aureus A3 growth, adhesion and biofilm formation was also limited by cell-free supernatant of L. acidophilus H-1 (BLIS negative). Moreover, competitive direct interactions were observed between staphylococci and the above bacteria, which influenced the formation of dualspecies aggregates on the surface.

• MeSH
• antibakteriální látky metabolismus   farmakologie   MeSH
• antibióza MeSH
• bakteriální adheze účinky léků   MeSH
• bakteriociny metabolismus   farmakologie   MeSH
• biofilmy účinky léků   MeSH
• kultivační média speciální farmakologie   MeSH
• Lactobacillus acidophilus růst a vývoj   metabolismus   fyziologie   MeSH
• lidé MeSH
• plankton růst a vývoj   MeSH
• probiotika MeSH
• Pseudomonas aeruginosa růst a vývoj   metabolismus   fyziologie   MeSH
• Staphylococcus aureus účinky léků   růst a vývoj   fyziologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• antibakteriální látky MeSH
• bakteriociny MeSH
• kultivační média speciální MeSH