The lincomycin biosynthetic gene lmbX was deleted in Streptomyces lincolnensis ATCC 25466, and deletion of this gene led to abolition of lincomycin production. The results of complementation experiments proved the blockage in the biosynthesis of lincomycin precursor 4-propyl-L-proline. Feeding this mutant strain with precursor derivatives resulted in production of 4'-butyl-4'-depropyllincomycin and 4'-pentyl-4'-depropyllincomycin in high titers and without lincomycin contamination. Moreover, 4'-pentyl-4'-depropyllincomycin was found to be more active than lincomycin against clinical Staphylococcus isolates with genes determining low-level lincosamide resistance.

• MeSH
• Anti-Bacterial Agents chemistry   metabolism   pharmacology   MeSH
• Bacterial Proteins genetics   metabolism   MeSH
• Humans MeSH
• Lincomycin analogs & derivatives   chemistry   metabolism   pharmacology   MeSH
• Microbial Sensitivity Tests MeSH
• Molecular Structure MeSH
• Proline analogs & derivatives   metabolism   MeSH
• Staphylococcal Infections microbiology   MeSH
• Staphylococcus drug effects   MeSH
• Streptomyces genetics   metabolism   MeSH
• Check Tag
• Humans MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Bacterial Proteins MeSH
• Lincomycin MeSH
• Proline MeSH