The Aleuria aurantia lectin (AAL) derived from orange peel fungus contains five fucose-binding sites that recognizes fucose bound in α-1,2, α-1,3, α-1,4, and α-1,6 linkages to N-acetylglucosamine and galactose. Recently, we have created several recombinant AAL (rAAL) proteins that had altered binding affinity to fucose linkages. In this report, we further characterize the binding specificity of one of the mutated lectins, N224Q lectin. This lectin was characterized by lectin Western blotting, surface plasmon resonance, and glycan microarray and shown to have increased binding to fucosylated glycan. Subsequently, we used this lectin to identify secreted fucosylated glycoproteins from a fetal hepatic cell line. Proteomic analysis revealed several glycoproteins secreted by the fetal cell line that were bound by N224Q lectin. These findings were confirmed by subsequent proteomic analysis of human serum from control patients or patients with hepatocellular carcinoma. These represent candidate oncofetal markers for liver cancer.

• Klíčová slova
• Aleuria aurantia lectin, Biomarker, Glycoproteomics, Glycosylation, Hepatocellular carcinoma, Liver cancer,
• MeSH
• Ascomycota chemie   MeSH
• buněčné linie MeSH
• fukosa analýza   metabolismus   MeSH
• glykoproteiny analýza   metabolismus   MeSH
• hepatocelulární karcinom diagnóza   metabolismus   MeSH
• hepatocyty metabolismus   patologie   MeSH
• játra metabolismus   patologie   MeSH
• kultivované buňky MeSH
• lektiny chemie   metabolismus   MeSH
• lidé MeSH
• nádorové biomarkery analýza   metabolismus   MeSH
• nádory jater diagnóza   metabolismus   MeSH
• polysacharidy chemie   metabolismus   MeSH
• proteomika MeSH
• rekombinantní proteiny chemie   metabolismus   MeSH
• vazba proteinů MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• fukosa MeSH
• glykoproteiny MeSH
• lectin, Aleuria aurantia MeSH Prohlížeč
• lektiny MeSH
• nádorové biomarkery MeSH
• polysacharidy MeSH
• rekombinantní proteiny MeSH