The extracellular ligninolytic enzyme system of Pleurotus laciniatocrenatus, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of Eucalyptus globulus wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1%) and peptone (0.5%), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 micromol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on E. globulus wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.

• MeSH
• biotechnologie metody   MeSH
• dřevo MeSH
• Eucalyptus metabolismus   MeSH
• kultivační média MeSH
• lakasa metabolismus   MeSH
• lignin metabolismus   MeSH
• peroxidasa metabolismus   MeSH
• Pleurotus enzymologie   růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Argentina MeSH
• Názvy látek
• kultivační média MeSH
• lakasa MeSH
• lignin MeSH
• peroxidasa MeSH

To evaluate the potential of using the enzymes from spent mushroom compost (SMC) as an industrial enzyme, the production of alpha-amylase, cellulase, beta-glucosidase, laccase, and xylanase was determined from the SMC of four edible mushroom species (Pleurotus ostreatus, Lentinula edodes, Flammulina velutipes and Hericium erinaceum). Among the tested SMC, the SMC of L. edodes showed the highest enzyme activity in alpha-amylase (229 nkat/g), cellulase (759 nkat/g) and beta-glucosidase (767 nkat/g) in 0.5% Triton X-100, and that of P. ostreatus showed the highest activity in laccase (1452 nkat/g) in phosphate-buffered 0.2% Triton X-100. The highest xylanase activity (119 nkat/g) was found in the SMC of F. velutipes.

• MeSH
• alfa-amylasy izolace a purifikace   metabolismus   MeSH
• Basidiomycota klasifikace   enzymologie   metabolismus   MeSH
• beta-glukosidasa izolace a purifikace   metabolismus   MeSH
• biodegradace MeSH
• celulasa izolace a purifikace   metabolismus   MeSH
• endo-1,4-beta-xylanasy izolace a purifikace   metabolismus   MeSH
• houby šii-take enzymologie   MeSH
• hydrolasy izolace a purifikace   metabolismus   MeSH
• lakasa izolace a purifikace   metabolismus   MeSH
• Pleurotus enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alfa-amylasy MeSH
• beta-glukosidasa MeSH
• celulasa MeSH
• endo-1,4-beta-xylanasy MeSH
• hydrolasy MeSH
• lakasa MeSH