The extracellular ligninolytic enzyme system of Pleurotus laciniatocrenatus, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of Eucalyptus globulus wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1%) and peptone (0.5%), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 micromol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on E. globulus wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.

lnstituto de Botainica Spegazzini Facultad de Ciencias Naturales y Museo Universidad Nacional de La Plata 1900 La Plata Argentina

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Appl Environ Microbiol. 1996 Mar;62(3):1070-2 PubMed

Curr Microbiol. 1997 Jan;34(1):1-5 PubMed

FEBS Lett. 1992 Mar 24;299(1):107-10 PubMed

Anal Biochem. 1976 May 7;72:248-54 PubMed

Arch Biochem Biophys. 2000 Nov 1;383(1):142-7 PubMed

Appl Environ Microbiol. 2002 Apr;68(4):1534-40 PubMed

Biodegradation. 1999 Jun;10(3):159-68 PubMed

Eur J Biochem. 1996 Apr 15;237(2):424-32 PubMed

Appl Environ Microbiol. 1997 Sep;63(9):3444-50 PubMed

Appl Environ Microbiol. 1993 Dec;59(12):4031-6 PubMed

Biochim Biophys Acta. 1991 Jan 23;1073(1):114-9 PubMed

FEBS Lett. 1998 May 29;428(3):141-6 PubMed

Rev Iberoam Micol. 2000 Jun;17(2):64-8 PubMed

Rapid Commun Mass Spectrom. 2002;16(1):62-8 PubMed

Rev Iberoam Micol. 1997 Sep;14(3):129-30 PubMed

Appl Environ Microbiol. 2000 Mar;66(3):920-4 PubMed

Folia Microbiol (Praha). 2003;48(4):525-8 PubMed

Mycologia. 2002 May-Jun;94(3):377-83 PubMed

Appl Microbiol Biotechnol. 2002 Apr;58(5):582-94 PubMed

Folia Microbiol (Praha). 2003;48(5):639-42 PubMed

Appl Environ Microbiol. 1999 Oct;65(10):4458-63 PubMed

Folia Microbiol (Praha). 2003;48(6):775-9 PubMed

Arch Biochem Biophys. 2000 Apr 1;376(1):171-9 PubMed

Folia Microbiol (Praha). 2003;48(3):413-6 PubMed

Biochim Biophys Acta. 1997 Apr 25;1339(1):23-30 PubMed

Appl Environ Microbiol. 2000 Feb;66(2):524-8 PubMed

J Biol Chem. 1998 Jun 19;273(25):15412-7 PubMed

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