Extracellular oxidative enzyme production and PAH removal in soil by exploratory mycelium of white rot fungi
Jazyk angličtina Země Nizozemsko Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem, Research Support, U.S. Gov't, Non-P.H.S.
PubMed
10492884
DOI
10.1023/a:1008324111558
Knihovny.cz E-zdroje
- MeSH
- anthraceny metabolismus MeSH
- Basidiomycota metabolismus MeSH
- biodegradace MeSH
- druhová specificita MeSH
- fenantreny metabolismus MeSH
- látky znečišťující půdu metabolismus MeSH
- lignin metabolismus MeSH
- oxidace-redukce MeSH
- Phanerochaete metabolismus MeSH
- Pleurotus metabolismus MeSH
- polycyklické aromatické uhlovodíky metabolismus MeSH
- Polyporales metabolismus MeSH
- půdní mikrobiologie MeSH
- pyreny metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Research Support, U.S. Gov't, Non-P.H.S. MeSH
- srovnávací studie MeSH
- Názvy látek
- anthracene MeSH Prohlížeč
- anthraceny MeSH
- fenantreny MeSH
- látky znečišťující půdu MeSH
- lignin MeSH
- phenanthrene MeSH Prohlížeč
- polycyklické aromatické uhlovodíky MeSH
- pyrene MeSH Prohlížeč
- pyreny MeSH
Selected strains of three species of white rot fungi, Pleurotus ostreatus, Phanerochaete chrysosporium and Trametes versicolor, were grown in sterilized soil from straw inocula. The respective colonization rates and mycelium density values decreased in the above mentioned order. Three- and four-ringed PAHs at 50 ppm inhibited growth of fungi in soil to some extent. The activities of fungal MnP and laccase (units per g dry weight of straw or soil), extracted with 50 mM succinate-lactate buffer (pH 4.5), were 5 to 20-fold higher in straw compared to soil. The enzyme activities per g dry soil in P. ostreatus and T. versicolor were similar, in contrast to P. chrysosporium, where they were extremely low. Compared to the aerated controls, P. ostreatus strains reduced the levels of anthracene, pyrene and phenanthrene by 81-87%, 84-93% and 41-64% within 2 months, respectively. During degradation of anthracene, all P. ostreatus strains accumulated anthraquinone. PAH removal rates in P. chrysosporium and T. versicolor soil cultures were much lower.
Citace poskytuje Crossref.org
Variability of laccase activity in the white-rot basidiomycete Pleurotus ostreatus