The aim of the study was to verify the hypothesis that a potential cause of the phytotoxicity of diclofenac (DCF, a non-steroidal anti-inflammatory drug) is an effect of cell cycle progression. This research was conducted using synchronous cultures of a model organism, green alga Chlamydomonas reinhardtii. The project examined DCF effects on selected parameters that characterize cell cycle progression, such as cell size, attainment of commitment points, DNA replication, number of nuclei formed during cells division and morphology of cells in consecutive stages of the cell cycle, together with the physiological and biochemical parameters of algae cells at different stages. We demonstrated that individual cell growth remained unaffected, whereas cell division was delayed in the DCF-treated groups grown in continuous light conditions, and the number of daughter cells from a single cell decreased. Thus, the cell cycle progression is a target affected by DCF, which has a similar anti-proliferative effect on mammalian cells.

• Klíčová slova
• Chlamydomonas reinhardtii, cell cycle, diclofenac, non-steroidal anti-inflammatory drug,
• MeSH
• antiflogistika nesteroidní toxicita   MeSH
• buněčné dělení účinky léků   MeSH
• buněčný cyklus účinky léků   MeSH
• Chlamydomonas reinhardtii účinky léků   genetika   růst a vývoj   MeSH
• diklofenak toxicita   MeSH
• DNA rostlinná biosyntéza   genetika   MeSH
• fotosyntéza účinky léků   MeSH
• replikace DNA účinky léků   MeSH
• velikost buňky účinky léků   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antiflogistika nesteroidní MeSH
• diklofenak MeSH
• DNA rostlinná MeSH