The aim of the study was to verify the hypothesis that a potential cause of the phytotoxicity of diclofenac (DCF, a non-steroidal anti-inflammatory drug) is an effect of cell cycle progression. This research was conducted using synchronous cultures of a model organism, green alga Chlamydomonas reinhardtii. The project examined DCF effects on selected parameters that characterize cell cycle progression, such as cell size, attainment of commitment points, DNA replication, number of nuclei formed during cells division and morphology of cells in consecutive stages of the cell cycle, together with the physiological and biochemical parameters of algae cells at different stages. We demonstrated that individual cell growth remained unaffected, whereas cell division was delayed in the DCF-treated groups grown in continuous light conditions, and the number of daughter cells from a single cell decreased. Thus, the cell cycle progression is a target affected by DCF, which has a similar anti-proliferative effect on mammalian cells.

Department of Plant Physiology and Biotechnology Faculty of Biology University of Gdansk Wita Stwosza 59 80 308 Gdańsk Poland

Department of Toxicology Medical University of Gdańsk Al Gen J Haller 107 80 416 Gdańsk Poland

Laboratory of Cell Cycles of Algae Centre Algatech Institute of Microbiology Czech Academy of Sciences Novohradská 237 379 01 Trebon Czech Republic

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