Synchronization of Green Algae by Light and Dark Regimes for Cell Cycle and Cell Division Studies
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
- Keywords
- Cell division, Chloroplast division, DNA replication, Green algae, Growth, Illumination regime, Multiple fission cell cycle, Nuclear division, Synchronization,
- MeSH
- Staining and Labeling methods MeSH
- Cell Division MeSH
- Cell Culture Techniques methods MeSH
- Cell Cycle MeSH
- Chlamydomonas reinhardtii cytology genetics growth & development MeSH
- Chlorophyta cytology genetics growth & development MeSH
- Chloroplasts genetics MeSH
- DNA, Plant genetics MeSH
- Photoperiod * MeSH
- Cell Fractionation methods MeSH
- DNA Replication MeSH
- Light MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- DNA, Plant MeSH
A synchronous population of cells is one of the prerequisites for studying cell cycle processes such as DNA replication, nuclear and cellular division. Green algae dividing by multiple fission represent a unique single cell system enabling the preparation of highly synchronous cultures by application of a light-dark regime similar to what they experience in nature. This chapter provides detailed protocols for synchronization of different algal species by alternating light-dark cycles; all critical points are discussed extensively. Moreover, detailed information on basic analysis of cell cycle progression in such cultures is presented, including analyses of nuclear, cellular, and chloroplast divisions. Modifications of basic protocols that enable changes in cell cycle progression are also suggested so that nuclear or chloroplast divisions can be followed separately.
References provided by Crossref.org
The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote
Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii
To Divide or Not to Divide? How Deuterium Affects Growth and Division of Chlamydomonas reinhardtii
Cell Cycle Arrest by Supraoptimal Temperature in the Alga Chlamydomonas reinhardtii
Response of the Green Alga Chlamydomonas reinhardtii to the DNA Damaging Agent Zeocin
