Cell growth and division processes are differentially sensitive to cadmium in Scenedesmus quadricauda
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
15058196
DOI
10.1007/bf02931518
Knihovny.cz E-zdroje
- MeSH
- buněčné dělení účinky léků MeSH
- buněčné jádro účinky léků MeSH
- chlorid kademnatý toxicita MeSH
- kultivační média farmakologie MeSH
- proteinkinasy metabolismus MeSH
- Scenedesmus cytologie účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- chlorid kademnatý MeSH
- histone H1 kinase MeSH Prohlížeč
- kultivační média MeSH
- proteinkinasy MeSH
The effect of cadmium on growth processes (accumulation of RNA, proteins and cell volume), cell cycle reproductive events (DNA replication, mitosis, protoplast fission and daughter-cell formation) and the regulatory activity of histone H1 kinases were monitored in synchronized cultures of the chlorococcal alga Scenedesmus quadricauda. Distinct dosage-dependent inhibitory effects of cadmium ions were found in individual growth and reproductive processes. At concentration of about 60 mumol/L CdCl2, the growth processes were slowed down after about half of the cell cycle but the cells grew to the same or larger size than did untreated cells. At higher concentration, the growth became progressively inhibited, being completely blocked above 240 mumol/L. Total RNA accumulation was the most sensitive growth process. Each of the reproductive events was a target for cadmium ions with increasing sensitivity in the following order: DNA replication, mitosis, protoplast fission and daughter cell formation. Throughout the entire experiment, the activity of "mitosis-specific" histone H1 kinases was negligible in the cadmium (60 mumol/L CdCl2) treated cultures, whilst that of the control culture varied, peaking just prior to nuclear divisions. The activity of "growth-associated" histone H1 kinases was not affected by cadmium ions. No effect was found if cadmium was present during the precommitment period. The longer the period in the presence of cadmium, the stronger inhibition of reproductive events.
Zobrazit více v PubMed
Arch Toxicol. 1990;64(5):383-6 PubMed
Microbiol Sci. 1984 Jul;1(4):96-101 PubMed
Plant Cell. 1989 Dec;1(12):1185-93 PubMed
Cell Biol Int. 1997 Jul;21(7):411-8 PubMed
Anal Biochem. 1976 Aug;74(2):448-56 PubMed
Toxicol Appl Pharmacol. 1997 Jun;144(2):225-34 PubMed
Folia Microbiol (Praha). 1999;44(4):406-10 PubMed
Cell Biol Toxicol. 1991 Jan;7(1):35-47 PubMed
Cell. 1989 Jul 28;58(2):361-72 PubMed
EMBO J. 1987 Nov;6(11):3507-14 PubMed
Plant Physiol. 2000 Jul;123(3):825-32 PubMed
Chem Res Toxicol. 2001 Sep;14(9):1193-202 PubMed
Folia Microbiol (Praha). 2001;46(3):227-30 PubMed
Diclofenac Alters the Cell Cycle Progression of the Green Alga Chlamydomonas reinhardtii
Cell Cycle Arrest by Supraoptimal Temperature in the Alga Chlamydomonas reinhardtii