Nejvíce citovaný článek - PubMed ID 15388748
Three bacterial strains producing blue-violet pigmented colonies on R2A agar were isolated from a wet rock wall and lakes in the deglaciated northern part of James Ross Island, Antarctica. The isolated strains inhibited phytopathogenic Gram-positive bacteria Clavibacter spp., Curtobacterium flacumfaciens, and Paenarthrobacter ilicis. Phylogenetic analysis based on the 16S rRNA gene indicated that the isolates belonged to the genus Massilia and the closest relatives were Massilia violaceinigra B2T, Massilia rubra CCM 8692T, Massilia frigida CCM 8695T, Massilia antarctica CCM 8941T, and Massilia aquatica CCM 8693T. A polyphasic taxonomic study based on lepA genes sequencing, automated ribotyping, MALDI-TOF MS, chemotaxonomy analyses, extensive biotyping, average nucleotide identity, and digital DNA-DNA hybridization calculations based on whole-genome sequences proved that the isolates represent a novel Massilia species for which the names Massilia pseudoviolaceinigra sp. nov. and Massilia scottii sp. nov. are suggested, with the type strains P3689T (= CCM 9206T = LMG 33568T) and P5043T (= CCM 9029T = LMG 32502T), respectively. These two bioactive metabolite-producing species may play an important role in shaping the composition of fresh-water Antarctic microbiomes due to the inhibition of various Gram-positive bacteria.
- Klíčová slova
- Clavibacter, Curtobacterium, Massilia, Antarctica, Plant-pathogenic, Taxonomic description,
- MeSH
- Actinobacteria MeSH
- biologická ochrana * izolace a purifikace metabolismus MeSH
- Clavibacter * patogenita MeSH
- DNA bakterií genetika izolace a purifikace MeSH
- extremofilové * klasifikace genetika izolace a purifikace metabolismus MeSH
- fylogeneze MeSH
- nemoci rostlin * mikrobiologie prevence a kontrola MeSH
- Oxalobacteraceae * klasifikace genetika izolace a purifikace metabolismus MeSH
- RNA ribozomální 16S genetika MeSH
- sekvenování celého genomu MeSH
- sladká voda mikrobiologie MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Antarktida MeSH
- Názvy látek
- biologická ochrana * MeSH
- DNA bakterií MeSH
- RNA ribozomální 16S MeSH
