Liposome-based drug delivery systems hold great potential for cancer therapy. The aim of this study was to design a nanodevice for targeted anchoring of liposomes (with and without cholesterol) with encapsulated anticancer drugs and antisense N-myc gene oligonucleotide attached to its surface. To meet this main aim, liposomes with encapsulated doxorubicin, ellipticine and etoposide were prepared. They were further characterized by measuring their fluorescence intensity, whereas the encapsulation efficiency was estimated to be 16%. The hybridization process of individual oligonucleotides forming the nanoconstruct was investigated spectrophotometrically and electrochemically. The concentrations of ellipticine, doxorubicin and etoposide attached to the nanoconstruct in gold nanoparticle-modified liposomes were found to be 14, 5 and 2 µg·mL(-1), respectively. The study succeeded in demonstrating that liposomes are suitable for the transport of anticancer drugs and the antisense oligonucleotide, which can block the expression of the N-myc gene.

• Klíčová slova
• N-myc, doxorubicin, ellipticine, etoposide, gold nanoparticles, liposome,
• MeSH
• antisense DNA chemie   terapeutické užití   MeSH
• doxorubicin chemie   terapeutické užití   MeSH
• elipticiny chemie   terapeutické užití   MeSH
• etoposid chemie   terapeutické užití   MeSH
• fluorescence MeSH
• lékové transportní systémy * MeSH
• lidé MeSH
• liposomy chemie   terapeutické užití   MeSH
• magnetické nanočástice chemie   terapeutické užití   MeSH
• nádory farmakoterapie   MeSH
• protoonkogen n-myc antagonisté a inhibitory   genetika   MeSH
• zlato chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antisense DNA MeSH
• doxorubicin MeSH
• elipticiny MeSH
• etoposid MeSH
• liposomy MeSH
• magnetické nanočástice MeSH
• protoonkogen n-myc MeSH
• zlato MeSH