Quercetin and phenylpropanoids are well known chemoprotective compounds identified in many plants. This study was aimed at determining their effects on activation of Nuclear factor erythroid 2-related factor 2 (Nrf2) antioxidant response element (Nrf2-ARE) signalling pathway and expression of its important downstream effector phase II detoxification enzyme glutathione-S-transferase P1 (GSTP1) in BJ foreskin fibroblasts and skin HaCaT keratinocytes. Cell lines and their corresponding Nrf2-ARE luciferase reporter cells were treated by ginger phenylpropanoids and quercetin for 10 h and the level of Nrf2 activity was subsequently determined. Both, ginger phenylpropanoids and quercetin, significantly increased the level of Nrf2 activity. Subsequent western blot analyses of proteins showed the increased expression level of glutathione-S-transferase P1 (GSTP1) in BJ cells but not in HaCaT cells. Such phenomenon of unresponsive downstream target expression in HaCaT cells was consistent with previous studies showing a constitutive expression of their GSTP1. Thus, while both ginger phenylpropanoids and quercetin have the property of increasing the level of Nrf2 both in HaCaT and in BJ cells, their effects on its downstream signalling were mediated only in BJ cells.

• MeSH
• antioxidancia aplikace a dávkování   chemie   MeSH
• buněčné linie MeSH
• faktor 2 související s NF-E2 biosyntéza   genetika   MeSH
• fibroblasty účinky léků   metabolismus   MeSH
• glutathion-S-transferasa fí biosyntéza   genetika   MeSH
• glutathiontransferasa MeSH
• keratinocyty účinky léků   metabolismus   MeSH
• lidé MeSH
• quercetin aplikace a dávkování   MeSH
• regulace genové exprese účinky léků   MeSH
• signální transdukce účinky léků   MeSH
• zázvor lékařský chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• faktor 2 související s NF-E2 MeSH
• glutathion-S-transferasa fí MeSH
• glutathiontransferasa MeSH
• leukotriene-C4 synthase MeSH Prohlížeč
• quercetin MeSH