Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus.

• MeSH
• adjuvancia imunologická aplikace a dávkování   izolace a purifikace   MeSH
• Bacillus chemie   MeSH
• infekce viry z čeledi Orthomyxoviridae prevence a kontrola   MeSH
• kultivované buňky MeSH
• lymfocyty účinky léků   MeSH
• modely nemocí na zvířatech MeSH
• myši MeSH
• proliferace buněk účinky léků   MeSH
• tvorba protilátek účinky léků   MeSH
• vakcíny proti chřipce aplikace a dávkování   MeSH
• virus chřipky A imunologie   MeSH
• virus chřipky B imunologie   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adjuvancia imunologická MeSH
• vakcíny proti chřipce MeSH

Twelve lactobacilli isolates from mucosa of 3-5-week-old weaned pigs were found to exert good antimicrobial activity against common porcine pathogens (S. aureus, B. cereus, E. coli, C. perfringens). Two of them produced in addition to lactic acid also considerable amounts of acetic acid, and 6 of them produced hydrogen peroxide and metabolites other than organic acids. Isolates 4/26 and 2/25 (identified as L. crispatus or L. amylovorus) were inhibitory against most strains of S. aureus, B. cereus and E. coli, and especially the strain 4/26 survived well in simulated gastric and intestinal juice. Diarrhea-causing E. coli O8K88H9 Ent(+) was successfully inhibited by the growing culture as well as by the catalase-treated and neutralized supernatant of L. reuteri 12/26. Mucin degradation and multiple resistance to antibiotics were not observed.

• MeSH
• antibiotická rezistence MeSH
• Bacillus cereus účinky léků   MeSH
• Clostridium perfringens účinky léků   MeSH
• Escherichia coli účinky léků   MeSH
• feces mikrobiologie   MeSH
• ileum mikrobiologie   MeSH
• kultivační média speciální farmakologie   MeSH
• kultivační média chemie   farmakologie   MeSH
• kyselina mléčná farmakologie   MeSH
• Lactobacillus účinky léků   izolace a purifikace   metabolismus   MeSH
• muciny metabolismus   MeSH
• nemoci prasat prevence a kontrola   MeSH
• odstavení MeSH
• peroxid vodíku metabolismus   MeSH
• přirozená imunita MeSH
• probiotika MeSH
• Staphylococcus aureus účinky léků   MeSH
• střevní sliznice mikrobiologie   MeSH
• Sus scrofa mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• kultivační média speciální MeSH
• kultivační média MeSH
• kyselina mléčná MeSH
• muciny MeSH
• peroxid vodíku MeSH