Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Language English Country United States Media print-electronic
Document type Journal Article, Research Support, Non-U.S. Gov't
- MeSH
- Adjuvants, Immunologic administration & dosage isolation & purification MeSH
- Bacillus chemistry MeSH
- Orthomyxoviridae Infections prevention & control MeSH
- Cells, Cultured MeSH
- Lymphocytes drug effects MeSH
- Disease Models, Animal MeSH
- Mice MeSH
- Cell Proliferation drug effects MeSH
- Antibody Formation drug effects MeSH
- Influenza Vaccines administration & dosage MeSH
- Influenza A virus immunology MeSH
- Influenza B virus immunology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Influenza Vaccines MeSH
Mucosal immunization with non-living antigens usually requires the use of an adjuvant. The adjuvant activity of Bacillus firmus in the mucosal immunization of mice was described by our laboratory previously. In the present study, subcellular localization of B. firmus activities was followed. After mechanical disintegration, subcellular components of bacterium were fractionated by differential centrifugation and salting out. Bacterial cell walls, cytoplasmic membrane fraction, soluble cytoplasmic proteins, and ribosomal fractions were isolated. Their effect on the mouse immune system was studied. Lymphocyte proliferation and immunoglobulin formation in vitro were stimulated by bacterial cell wall (BCW), cytoplasmic membrane (CMF), and ribosomal fractions. BCW and CMF increased antibody formation after intratracheal immunization of mice with influenza A and B viruses, and increased protection against subsequent infection with influenza virus. The BCW fraction even induced intersubtypic cross-protection: Mice immunized with A/California/7/04 (H3N2) + BCW were resistant to the infection by the highly pathogenic A/PR/8/34 (H1N1) virus.
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