Effect of Bacillus firmus on antibody formation after mucosal and parenteral immunization in mice
Language English Country Netherlands Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
9870668
DOI
10.1016/s0165-2478(98)00105-9
PII: S0165-2478(98)00105-9
Knihovny.cz E-resources
- MeSH
- Adjuvants, Immunologic MeSH
- Antigens, Bacterial administration & dosage immunology MeSH
- Administration, Oral MeSH
- Administration, Rectal MeSH
- Bacillus immunology MeSH
- Immunization * MeSH
- Immunoglobulins blood MeSH
- Injections, Intraperitoneal MeSH
- Injections, Subcutaneous MeSH
- Mice MeSH
- Ovalbumin administration & dosage immunology MeSH
- Antibodies, Bacterial blood MeSH
- Intestine, Small immunology MeSH
- Antibody Formation * MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Adjuvants, Immunologic MeSH
- Antigens, Bacterial MeSH
- Immunoglobulins MeSH
- Ovalbumin MeSH
- Antibodies, Bacterial MeSH
Immunostimulatory properties of B. firmus, a nontoxic, nonpathogenic G + bacterium of external environment, were described previously. Antiinfectious and antitumor activity, macrophage activation and strong polyclonal stimulation of B lymphocytes were proved in human, mice and rats. The adjuvant effect of B. firmus on specific antibody response to ovalbumin in BALB/c mice is the topic of the present study. Against our expectation, B. firmus exerts more suppressive than stimulatory effect on specific antibody response. Formolized B. firmus decreased anti-ovalbumin response after subcutaneous immunization and only slightly increased serum antibodies after intraperitoneal immunization. After mucosal immunization, both oral and rectal, ovalbumin itself did not cause a significant systemic response but induced IgA anti-ovalbumin response in the intestine. B. firmus applied together with ovalbumin increased systemic serum response but absolutely eliminated intestinal response. The rectal route of antigen administration has been found less convenient because of less precise dosing of antigen in this mode of immunization.
References provided by Crossref.org
Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Immunomodulatory effects of Bacillus firmus on mouse peritoneal cells in vitro
Immunostimulatory effect of Bacillus firmus on mouse lymphocytes
