Immunostimulatory effect of Bacillus firmus on mouse lymphocytes
Jazyk angličtina Země Spojené státy americké Médium print
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
12058402
DOI
10.1007/bf02817682
Knihovny.cz E-zdroje
- MeSH
- aktivace lymfocytů * účinky léků MeSH
- B-lymfocyty účinky léků imunologie MeSH
- Bacillus chemie imunologie MeSH
- ELISA MeSH
- imunoglobulin G biosyntéza účinky léků MeSH
- kultivované buňky MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- protilátky bakteriální biosyntéza MeSH
- T-lymfocyty imunologie MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- imunoglobulin G MeSH
- protilátky bakteriální MeSH
Bacillus firmus (a Gram-positive nonpathogenic and harmless bacterium), was shown to be a strong polyclonal activator of mouse B lymphocytes as estimated by ELISA testing of Ig concentrations in culture supernatants after incubation of BALB/c mouse splenocytes with inactivated bacillus. Synthesis of all main Ig classes and all IgG subclasses was stimulated in vitro, the considerable effect on IgA formation being the most interesting feature. B cell stimulation was T cell dependent, as was demonstrated by the effect of B. firmus on all Ig isotypes and by comparison of lymphocyte response of nu/nu mice and heterozygous nu/+ mice. The effect of B. firmus on splenocyte proliferation was stimulatory or suppressive depending on the dose of the bacterium. Increased synthesis of IFN-gamma and IL-10 (detected by ELISA in splenocyte culture supernatants) showed probable stimulation of Th1 and Th2 subpopulations. Considering the stimulatory effect on IgA formation and macrophage stimulation, B. firmus seems to be a prospective mucosal adjuvant and/or probiotic.
Zobrazit více v PubMed
Folia Microbiol (Praha). 1992;37(6):455-60 PubMed
Methods Enzymol. 1984;108:88-102 PubMed
Immunol Lett. 1998 Dec;64(2-3):161-6 PubMed
Annu Rev Immunol. 1989;7:145-73 PubMed
Clin Immunol Immunopathol. 1997 Sep;84(3):244-50 PubMed
Immunol Today. 1990 Jun;11(6):200-3 PubMed
Folia Microbiol (Praha). 1994;39(2):147-51 PubMed
Immunol Lett. 1991 Mar;27(3):225-30 PubMed
Vaccine. 1997 Feb;15(3):248-56 PubMed
Folia Microbiol (Praha). 1994;39(6):501-4 PubMed
Int J Immunopharmacol. 1998 Jul;20(7):359-68 PubMed
Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Probiotics manipulate host cytokine response and induce antimicrobial peptides
Immunomodulatory effects of Bacillus firmus on mouse peritoneal cells in vitro
