Stimulation of macrophages by Bacillus firmus: production of nitric oxide and cytokines
Jazyk angličtina Země Anglie, Velká Británie Médium print
Typ dokumentu srovnávací studie, časopisecké články, práce podpořená grantem
PubMed
9756131
DOI
10.1016/s0192-0561(98)00025-3
PII: S0192056198000253
Knihovny.cz E-zdroje
- MeSH
- aktivace makrofágů * MeSH
- Bacillus imunologie MeSH
- interferon gama analýza farmakologie MeSH
- interleukin-10 antagonisté a inhibitory imunologie metabolismus MeSH
- interleukin-2 analýza MeSH
- lipopolysacharidy farmakologie MeSH
- monoklonální protilátky imunologie farmakologie MeSH
- myši inbrední C57BL MeSH
- myši MeSH
- Nocardia imunologie MeSH
- oxid dusnatý biosyntéza MeSH
- peritoneální makrofágy metabolismus MeSH
- rekombinantní proteiny MeSH
- synthasa oxidu dusnatého metabolismus MeSH
- TNF-alfa antagonisté a inhibitory imunologie metabolismus MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- interferon gama MeSH
- interleukin-10 MeSH
- interleukin-2 MeSH
- lipopolysacharidy MeSH
- monoklonální protilátky MeSH
- oxid dusnatý MeSH
- rekombinantní proteiny MeSH
- synthasa oxidu dusnatého MeSH
- TNF-alfa MeSH
Immunostimulatory properties of gram-positive Bacillus firmus were investigated under in vitro conditions using murine peritoneal macrophages. B. firmus stimulated in a concentration and time dependent manner the secretion of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10), but it had no influence upon interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) production. It also substantially augmented production of nitric oxide (NO) induced by exogenous IFN-gamma. Inhibitory experiments using neutralizing antibodies against TNF-alpha and/or IL-10 have demonstrated that these cytokines are responsible for triggering the underlying mechanism(s) leading to enhanced NO production. The cytokine-stimulatory and NO-costimulatory properties could participate in the antiinfectious and anticancer effects of B. firmus, detected previously in the in vivo experiments.
Citace poskytuje Crossref.org
Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Immunomodulatory effects of Bacillus firmus on mouse peritoneal cells in vitro
Immunostimulatory effect of Bacillus firmus on mouse lymphocytes
