Stimulation of macrophages by Bacillus firmus: production of nitric oxide and cytokines
Language English Country England, Great Britain Media print
Document type Comparative Study, Journal Article, Research Support, Non-U.S. Gov't
PubMed
9756131
DOI
10.1016/s0192-0561(98)00025-3
PII: S0192056198000253
Knihovny.cz E-resources
- MeSH
- Macrophage Activation * MeSH
- Bacillus immunology MeSH
- Interferon-gamma analysis pharmacology MeSH
- Interleukin-10 antagonists & inhibitors immunology metabolism MeSH
- Interleukin-2 analysis MeSH
- Lipopolysaccharides pharmacology MeSH
- Antibodies, Monoclonal immunology pharmacology MeSH
- Mice, Inbred C57BL MeSH
- Mice MeSH
- Nocardia immunology MeSH
- Nitric Oxide biosynthesis MeSH
- Macrophages, Peritoneal metabolism MeSH
- Recombinant Proteins MeSH
- Nitric Oxide Synthase metabolism MeSH
- Tumor Necrosis Factor-alpha antagonists & inhibitors immunology metabolism MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Female MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Comparative Study MeSH
- Names of Substances
- Interferon-gamma MeSH
- Interleukin-10 MeSH
- Interleukin-2 MeSH
- Lipopolysaccharides MeSH
- Antibodies, Monoclonal MeSH
- Nitric Oxide MeSH
- Recombinant Proteins MeSH
- Nitric Oxide Synthase MeSH
- Tumor Necrosis Factor-alpha MeSH
Immunostimulatory properties of gram-positive Bacillus firmus were investigated under in vitro conditions using murine peritoneal macrophages. B. firmus stimulated in a concentration and time dependent manner the secretion of tumour necrosis factor-alpha (TNF-alpha) and interleukin-10 (IL-10), but it had no influence upon interferon-gamma (IFN-gamma) and interleukin-2 (IL-2) production. It also substantially augmented production of nitric oxide (NO) induced by exogenous IFN-gamma. Inhibitory experiments using neutralizing antibodies against TNF-alpha and/or IL-10 have demonstrated that these cytokines are responsible for triggering the underlying mechanism(s) leading to enhanced NO production. The cytokine-stimulatory and NO-costimulatory properties could participate in the antiinfectious and anticancer effects of B. firmus, detected previously in the in vivo experiments.
References provided by Crossref.org
Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
Immunomodulatory effects of Bacillus firmus on mouse peritoneal cells in vitro
Immunostimulatory effect of Bacillus firmus on mouse lymphocytes
