Immunostimulatory effect of Bacillus firmus on mouse lymphocytes
Language English Country United States Media print
Document type Journal Article, Research Support, Non-U.S. Gov't
PubMed
12058402
DOI
10.1007/bf02817682
Knihovny.cz E-resources
- MeSH
- Lymphocyte Activation * drug effects MeSH
- B-Lymphocytes drug effects immunology MeSH
- Bacillus chemistry immunology MeSH
- Enzyme-Linked Immunosorbent Assay MeSH
- Immunoglobulin G biosynthesis drug effects MeSH
- Cells, Cultured MeSH
- Mice, Inbred BALB C MeSH
- Mice MeSH
- Antibodies, Bacterial biosynthesis MeSH
- T-Lymphocytes immunology MeSH
- Animals MeSH
- Check Tag
- Mice MeSH
- Animals MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
- Names of Substances
- Immunoglobulin G MeSH
- Antibodies, Bacterial MeSH
Bacillus firmus (a Gram-positive nonpathogenic and harmless bacterium), was shown to be a strong polyclonal activator of mouse B lymphocytes as estimated by ELISA testing of Ig concentrations in culture supernatants after incubation of BALB/c mouse splenocytes with inactivated bacillus. Synthesis of all main Ig classes and all IgG subclasses was stimulated in vitro, the considerable effect on IgA formation being the most interesting feature. B cell stimulation was T cell dependent, as was demonstrated by the effect of B. firmus on all Ig isotypes and by comparison of lymphocyte response of nu/nu mice and heterozygous nu/+ mice. The effect of B. firmus on splenocyte proliferation was stimulatory or suppressive depending on the dose of the bacterium. Increased synthesis of IFN-gamma and IL-10 (detected by ELISA in splenocyte culture supernatants) showed probable stimulation of Th1 and Th2 subpopulations. Considering the stimulatory effect on IgA formation and macrophage stimulation, B. firmus seems to be a prospective mucosal adjuvant and/or probiotic.
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