Protective and cross-protective mucosal immunization of mice by influenza virus type A with bacterial adjuvant
Jazyk angličtina Země Nizozemsko Médium print-electronic
Typ dokumentu časopisecké články, práce podpořená grantem
PubMed
18160106
DOI
10.1016/j.imlet.2007.11.001
PII: S0165-2478(07)00285-4
Knihovny.cz E-zdroje
- MeSH
- adjuvancia imunologická * MeSH
- Bacillus imunologie MeSH
- chřipka lidská imunologie mortalita prevence a kontrola virologie MeSH
- cytokiny biosyntéza imunologie MeSH
- imunizace MeSH
- infekce viry z čeledi Orthomyxoviridae imunologie mortalita prevence a kontrola MeSH
- lidé MeSH
- myši inbrední BALB C MeSH
- myši MeSH
- plíce imunologie patologie MeSH
- protilátky virové krev imunologie MeSH
- slizniční imunita MeSH
- vakcíny proti chřipce imunologie MeSH
- virus chřipky A, podtyp H1N1 imunologie MeSH
- virus chřipky A, podtyp H3N2 imunologie MeSH
- zkřížené reakce MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- myši MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- adjuvancia imunologická * MeSH
- cytokiny MeSH
- protilátky virové MeSH
- vakcíny proti chřipce MeSH
Mucosal immunization by inactivated viruses often fails to evoke a sufficient immune response. Intensive efforts have been made to enhance the response by suitable adjuvants. We used the G+ nonpathogenic delipidated bacterium Bacillus firmus with pronounced immunostimulatory properties as an adjuvant for immunizing mice with inactivated influenza virus type A. BALB/c mice were immunized intratracheally with inactivated influenza A H1N1 and H3N2 viruses. The production of antibodies in sera and secretions was determined by the ELISA. The local situation in the lungs was assessed histologically and by testing the cytokine expression. The protective and cross-protective effect against infection was tested in in vivo experiments after infection with influenza virus A H1N1. B. firmus as adjuvant increased both systemic and mucosal antibody responses, improved protection against homologous virus and induced cross-protection against virus H1N1 after immunization with virus H3N2.
Citace poskytuje Crossref.org
Immunomodulatory properties of subcellular fractions of a G+ bacterium, Bacillus firmus
