White rot fungi (WRF) are applicable to biodegradation of recalcitrant pollutants. However, excessive biomass growth typical for WRF cultivation can hinder their large scale applications. Therefore, immobilization of Irpex lacteus to liquid-core alginate beads restricting excessive mycelium growth and simultaneously keeping high degradation rate of pollutants was tested. Effective diffusivities of dyes to the beads varied from (2.98 ± 0.69) × 10-10 to (10.27 ± 2.60) × 10-10 m2/s. Remazol Brilliant Blue R (RBBR), Reactive Orange 16 (RO16), and Naphthol Blue Black (NBB) were used as model dyes. The immobilized fungus decolorized model dyes when applied both in microwell plates and in fluidized bed reactors. Using the microwell plates, the apparent reaction rate constants ranged from (2.06 ± 0.11) × 10-2 to (11.06 ± 0.27) × 10-2 1/h, depending on the dye used and its initial concentration. High initial concentrations negatively affected the dye decolorization rate. No fungal growth outside the beads was observed in fluidized bed reactors and thus no operational problems linked to an excessive biomass growth occurred. When RBBR was decolorized in subsequent batches in the fluidized bed reactor, the apparent reaction rate constant increased from (11.63 ± 0.35) × 10-2 to (29.26 ± 7.19) × 10-2 1/h.

• MeSH
• Alginates chemistry   MeSH
• Coloring Agents metabolism   MeSH
• Biodegradation, Environmental MeSH
• Water Pollutants, Chemical metabolism   MeSH
• Cells, Immobilized chemistry   metabolism   MeSH
• Glucuronic Acid chemistry   MeSH
• Hexuronic Acids chemistry   MeSH
• Mycelium chemistry   growth & development   metabolism   MeSH
• Polyporales chemistry   growth & development   metabolism   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Alginates MeSH
• Coloring Agents MeSH
• Water Pollutants, Chemical MeSH
• Glucuronic Acid MeSH
• Hexuronic Acids MeSH

Three new chromatographic forms of Dichomitus squalens manganese-dependent peroxidase (MnP) were isolated from wheat-straw cultures using Mono Q and connective interaction media (CIM) fast protein liquid chromatography. Enzymes revealed identical molar mass of 50 kDa (estimated by SDS-PAGE) and pI values of 3.5, however, they varied in Km values obtained for Mn2+ oxidation. The addition of wood and straw methanol extracts to the cultures showed that the production of MnPs in wheat-straw cultures was influenced rather by the type of cultivation than by phenolic compounds from lignocellulosic material which induced laccase production. The purified CIM1 MnP was able to decolorize selected azo and anthraquinone dyes more rapidly than laccase Lc1. In vitro dye decolorization showed a synergistic cooperation of MnP and laccase. In the case of CSB degradation MnP prevented from the production of a differently colored substance that could be produced after CSB degradation by laccase-HBT system.

• MeSH
• Anthraquinones metabolism   MeSH
• Azo Compounds metabolism   MeSH
• Bacterial Proteins isolation & purification   metabolism   MeSH
• Color MeSH
• Coloring Agents metabolism   MeSH
• Electrophoresis, Polyacrylamide Gel MeSH
• Laccase isolation & purification   metabolism   MeSH
• Lignin metabolism   MeSH
• Molecular Weight MeSH
• Mycology methods   MeSH
• Naphthalenesulfonates metabolism   MeSH
• Oxidation-Reduction MeSH
• Peroxidases isolation & purification   metabolism   MeSH
• Polyporaceae enzymology   MeSH
• Triticum MeSH
• Drug Synergism MeSH
• Trypan Blue metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Comparative Study MeSH
• Names of Substances
• Anthraquinones MeSH
• Azo Compounds MeSH
• Bacterial Proteins MeSH
• Coloring Agents MeSH
• C.I. Reactive Violet 5 MeSH Browser
• Laccase MeSH
• Lignin MeSH
• lignocellulose MeSH Browser
• manganese peroxidase MeSH Browser
• Naphthalenesulfonates MeSH
• Peroxidases MeSH
• pontamine sky blue MeSH Browser
• reactive orange 16 MeSH Browser
• Remazol Brilliant Blue R MeSH Browser
• Trypan Blue MeSH